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DNA Topoisomerases

DNA Topoisomerases


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About the Book

Preface...
Table of Contents...
Contributing Authors...

1- Type IA DNA Topoisomerases: A Universal Core and Multiple Activities
Florence Garnier, Hélène Debat, and Marc Nadal

2- Topoisomerase I and Genome Stability: The Good and The Bad
Jang-Eun Cho and Sue Jinks-Robertson

3- DNA Topoisomerases as Targets for Antibacterial Agents
Hiroshi Hiasa

4- DNA Supercoiling Measurement in Bacteria
Yingting Liu, Zhi-Chun Hua, and Fenfei Leng

5- DNA Catenation Reveals the Dynamics of DNA Topology during Replication
Alicia Castán, Pablo Hernández, Dora B. Krimer, and Jorge B. Schvartzman

6- Mapping E. coli Topoisomerase IV Binding and Activity Sites
Hafez El Sayyed and Olivier Espéli

7- The Use of Psoralen Photobinding to Study Transcription-Induced Supercoiling
Fedor Kouzine, Laura Baranello, and David Levens

8- Immunoprecipitation of RNA: DNA Hybrids from Budding Yeast
Aziz El Hage and David Tollervey

9- Detect

ion of oriC-Independent Replication in Escherichia coli Cells
Makisha Martel, Aurélien Balleydier, Julien Brochu, and Marc Drolet

10- Single-Molecule Magnetic Tweezer Analysis of Topoisomerases
Kathryn H. Gunn, John F. Marko, and Alfonso Mondragón

11- Synthesis of Hemicatenanes for the Study of Type IA Topoisomerases
Shun-Hsiao Lee, Tao-shih Hsieh, and Grace Ee-Lu Siaw

12- An Assay for Detecting RNA Topoisomerase Activity
Muzammil Ahmad, Dongyi Xu, and Weidong Wang

13- Studying TDP1 Function in DNA Repair
Shih-Chieh Chiang, Kirsty Liversidge, and Sherif F. El-Khamisy

14- Topoisomerase II Chromatin Immunoprecipitation
Kayleigh A. Smith, Ian G. Cowell, and Caroline A. Austin

15- Analyzing Mitotic Chromosome Structural Defects after Topoisomerase II Inhibition or Mutation
Juan F. Giménez-Abián, Andrew B. Lane, and Duncan J. Clarke

16- Monitoring the DNA Topoisomerase II Checkpoint in Saccharomyces cerevisiae
Katherine Furniss, Amit C. J. Vas, Andrew Lane, and Duncan J. Clarke

17- Studying Topoisomerase 1-Mediated Damage at Genomic Ribonucleotides
Jessica S. Williams and Thomas A. Kunkel

18- A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
Thirunavukkarasu Annamalai, Bokun Cheng, and Yuk-Ching Tse-Dinh

19- Fluoroquinolone-Gyrase-DNA Cleaved Complexes
Gan Luan and Karl Drlica

20- Detection of Topoisomerase Covalent Complexes in Eukaryotic Cells
Jay Anand, Yilun Sun, Yang Zhao, Karin C. Nitiss, and John L. Nitiss

21- Visualization and Quantification of Topoisomerase-DNA Covalent Complexes Using the Trapped in Agarose Immunostaining (TARDIS) Assay
Ian G. Cowell and Caroline A. Austin

22- Study of Plasmid-Mediated Quinolone Resistance in Bacteria
George A. Jacoby



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Product Details
  • ISBN-13: 9781493974580
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: 1st ed. 2018
  • Language: English
  • Returnable: Y
  • Spine Width: 21 mm
  • Weight: 861 gr
  • ISBN-10: 1493974580
  • Publisher Date: 25 Nov 2017
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 328
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm


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