1. Conventional Specimen Preparation Techniques For Transmission Electron Microscopy of Cultured Cells
John J. Bozzola
2. Microwave-assisted Processing and Embedding for Transmission Electron Microscopy
Paul Webster
3. Processing Plant Tissues for Ultrastructural Study John Kuo
4. Staining Sectioned Biological Specimens for Transmission Electron Microscopy:
Conventional and En Bloc Stains
E. Ann Ellis
5. Metal Shadowing for Electron Microscopy
Gregory M. Hendricks
6. Freeze Fracture and Freeze Etching
Douglas E. Chandler and William P. Sharp
7. Conventional Specimen Preparation Techniques For Scanning Electron Microscopy of Biological Specimens
John J. Bozzola
8. High-pressure Freezing: Current State and Future Prospects
Andres Kaech and Urs Ziegler
9. Cryo-fixation by Self-pressurized Rapid Freezing Markus Grabenbauer, Hong-Mei Han, and Jan Huebinger
10. Cryo-Electron Microscopy of Vitreous Sections
Petr Chlanda and Martin Sachse
11. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine
J. Robin Harris
12. Electron Microscopy of the Microtubule Cytoskeleton Assembly Vitro
Margaret Coughlin, Aaron C. Groen, Timothy J. Mitchison
13. Cryosectioning Fixed and Cryoprotected Biological Material for Immmunocytochemistry
Paul Webster and Alexandre Webster
14. Analysis of Specificity in Immunoelectron Microscopy
Christian Hacker and John M. Lucocq
15. Cryo-Electron Microscopy of Membrane Proteins
Kenneth N. Goldie, Priyanka Abeyrathne, Fabian Kebbel, Mohamed Chami, Philippe Ringler, and Henning Stahlberg
16. Tracking DNA and RNA Sequences at High Resolution
Dusan Cmarko, Anna Ligasová, and Karel Koberna
17. Visualization of DNA and Protein-DNA Complexes with Atomic Force Microscopy
Yuri L. Lyubchenko, Alexander A. Gall, and Luda S. Shlyakhtenko
18. Biological Applications of Phase-Contrast Electron Microscopy
Kuniaki Nagayama
19. Single Particle Cryo-Electron Microscopy And 3-D Reconstruction Of Viruses
Fei Guo and Wen Jiang
20. Electron Tomography for Organelles, Cells and Tissues
Wanzhong He and Yongning He
21. Correlative Light and Electron Microscopy: from Live Cell Dynamic to 3D Ultrastructure
Coralie Spiegelhalter, Jocelyn F. Laporte, and Yannick Schwab
22. Nanometer-resolution Fluorescence Electron Microscopy (Nano-EM) in Cultured Cells
Shigeki Watanabe, Martin Lehmann, Edward Hujber, Richard D. Fetter, Jackson Richards, Berit Söhl-Kielczynski, Annegret Felies, Christian Rosenmund, Jan Schmoranzer, and Erik M. Jorgensen
23. Correlative Fluorescence- and Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid
Diana B. Peckys, Madeline J. Dukes, and Niels de Jonge
24. FIB-SEM Tomography in Biology
Caroline Kizilyaprak
25. Correlative Light and Electron Microscopy using Immunolabeled Sections
Heinz Schwarz and Bruno M. Humbel
26. Correlative 3D Imaging: CLSM and FIB-SEM Tomography Using High-Pressure Frozen, Freeze-Substituted Biological Samples
Miriam S. Lucas, Maja Guenthert, Philippe Gasser, Falk Lucas and Roger Wepf
27. Three-dimensional Imaging of Adherent Cells using FIB-SEM and STEM
Clarissa Villinger, Martin Schauflinger, Heiko Gregorius, Christine Kranz, Katharina Höhn, Soufi Nafeey, Paul Walther
28. X-Ray Microanalysis in the Scanning Electron MicroscopeGodfried M. Roomans and Anca Dragomir
29. Application of SEM and EDX in Studying Biomineralization in Plant Tissues
Honghua He
30. Freeze Stabilization and Cryopreparation Technique for Visualizing the Water Distribution in Woody Tissues by X-ray Im
