Part I. Basic Concepts and Enhancement of DNA Transfer via Electroporation
1. Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments
Lluis M. Mir
2. Electropermeabilization of the Cell Membrane
Justin Teissié
3. The Impact of Non-Electrical Factors on Electrical Gene Transfer
Jiemiao Hu, Jeffry Cutrera, and Shulin Li
4. Electroporation Formulation for Cell Therapy
Jiemiao Hu and Shulin Li
5. Short-Fragment DNA-Mediated in Vivo DNA Electroporation Delivery
Jinliang Peng, Yonggang Zhao, and Yuhong Xu
6. Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection
Shuyan Huang, Yingbo Zu, and Shengnian Wang
Part II. SiRNA Electrotransfer
7. SiRNA Delivery via Electropulsation: A Review of the Basic Processes
Muriel Golzio and Justin Teissié
8. Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips
Zewen Wei and Zhihong Li
9. Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells
Isabel Popp, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan, and Jonathan Jantsch
10. Direct Imaging of siRNA Electrotransfer at the Single Cell Level
Justin Teissié and Muriel Golzio
11. Electroporation-Mediated siRNA Delivery into Tumors
Yoshifumi Takei
12. Substrate-Mediated, High Efficiency siRNA Electroporation
Hiroyuki Fujimoto and Hiroo Iwata
13. Intradermal Electroporation of RNA
Maria L. Knudsen, Karl Ljungberg, Peter Liljeström, and Daniel X. Johansson
Part III. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues
14. Delivery of DNA into the Central Nervous System via Electroporation
Jochen De Vry, Tim Vanmierlo, Pilar Martínez-Martínez, Mario Losen, Yasin Temel, Harry Steinbusch, Marc De Baets, and Jos Prickaerts
15. DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes
John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal, and Jeffrey H. Boatright
16. Delivery of Plasmid DNA into Developing Rat Teeth by Electroporation
Shaomian Yao, Michael L. Beckley, and Dawen Liu
17. Electroporation-Mediated Gene Delivery to the Lungs
Jennifer L. Young, Michael S. Barravecchia, and David A. Dean
18. Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion
David Machado-Aranda and Krishnan Raghavendran
19. In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart
Hendrik T. Tevaearai, Amiq Gazdhar, M. N. Giraud, and Martin Flück
Part IV. Treatment of Cancer in Large Animals and Humans via Electroporation Therapy
20. Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy
Jeffry Cutrera, Glenn King, Pamela Jones, Elias Gumpel, Xueqing Xia, and Shulin Li
21. Gene Electrotransfer in Clinical Trials
Julie Gehl
22. Electrochemotherapy in Veterinary Oncology: From Rescue to First-line Therapy
Enrico P. Spugnini and Alfonso Baldi
23. Low Energy DC Current Ablation in a Mouse Tumor Model
Prejesh Philips and Robert C. G. Martin
Part V. Applications of Electroporation for DNA Vaccination
24. Evolution of Electroporated DNA Vaccines
Andrea Keane-Myers and Matthew Bell
25. Clinical Development of Intramuscular Electroporation: Providing a "Boost" for DNA Vaccines
Amir S. Khan, Kate E. Broderick, and Niranjan Y. Sardesai
26. Electroporation-Mediated
