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Engineering Hexuronic Acid Catabolism

Engineering Hexuronic Acid Catabolism


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About the Book

Metabolic engineering targets for the production of high-value compounds. Citrus peel is produced 15,000,000 t per year worldwide causing environmental problems. Its main constituent is D-galacturonic acid and can be converted to useful chemicals using genetically engineered strains. Engineered Aspergillus niger were used in solid and submerged state fermentation to convert orange peel to L-galactonate in a consolidate process. The gaaB coding for L-galactonate dehydrogenase was deleted (ΔgaaB) and ii) the gaaB was deleted and the gaaA coding for D-galacturonate reductase was overexpressed (ΔgaaB-gaaA). ΔgaaB was able to convert up to 87 % of D-galacturonic acid to L-galactonate. Also, in this work, the eukaryotic D-Glucuronic acid pathway was studied. A decarboxylase that converts 3-keto-L-gulonate to L-xylulose remains poorly characterized and the gene is not known. A coupled enzyme assay was used to detect its activity. In the assay, ammonium sulfate precipitates from bovine liver extract were coupled with engineered L-gulonate-3-dehydrogenase and L-xylulose reductase. A 3-keto-L-gulonate decarboxylase activity could not be detected.


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Product Details
  • ISBN-13: 9783659580345
  • Publisher: Lap Lambert Academic Publishing
  • Publisher Imprint: LAP LAMBERT Academic Publishing
  • Height: 229 mm
  • No of Pages: 68
  • Spine Width: 4 mm
  • Width: 152 mm
  • ISBN-10: 3659580341
  • Publisher Date: 06 Aug 2014
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 163 gr


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Engineering Hexuronic Acid Catabolism
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Engineering Hexuronic Acid Catabolism
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