About the Book
PaPart I Tropisms
1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient
Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez
2. Assessing Gravitropic Responses in Arabidopsis
Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson
3. Physiological Analysis of Phototropic Responses in Arabidopsis
Mathias Zeidler
4. Automatic Chloroplast Movement Analysis
Henrik Johansson and Mathias Zeidler
5. Micro
scopic and Biochemical Visualization of Auxins in Plant Tissues
Joshua J. Blakeslee and Angus S. Murphy
6. Immunolocalization of PIN and ABCB Transporters in Plants
Nicola Carraro and Wendy A. Peer
Part II Photoperiodism and Circasian Rhythms
7.
Analysis of Circadian Leaf Movements
Niels A. Müller and José M. Jimémez-Gó
mez
8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes
Fernando Andrés, Stefano Torti, Coral Vincent, and George Coupland
9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are
Temporally Restricted by the Circadian Clock
Amaury de Montaigu, Markus C. Berns, and Geroge Coupland
10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome
Ghislain Breton, Steve A. Kay, and José L. Pruneda-Paz
11. Monitoring Alternative Splicing Changes in
Arabidopsis Circadian Clock Genes
Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger
12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock
Michael J. Haydon and Alex A. R. Webb
13. Assessing Protein Stability Under Different Light and Circadian Conditions
Takatoshi Kiba and Rossana Henriques
Part III Abiotic Stress Responses
14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought
Diego M. Almeida, M. Cecília Almadanim, Tiago Louren
ç
o, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira
15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana
Urszula Piskurewicz and Luis Lopez-Molina
16. Assessing Tolerance to Heavy-Metal Stress in
Arabidopsis Thaliana Seedings
Estelle Remy and Paula Duque
17. Assessing Drought Responses Using Thermals Infrared Imaging
Ankush Prashar and Hamlyn G. Jones
18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms
Monika Maronova and Maria Kalyna
19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures
Imma P. Salamó, Bogáta Boros, and L
ászló Szabados
20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress
Ana Confraria and Elena Baena-González
21. Construction of Artificial miRNAs to Prevent Drought Stress in
Solanum Tuberosum
Anna Wyrzykowska, Marcin Pieczyński, and Zofia Szweykowska-Kuli
About the Author: Paula Duque Plant Molecular Biology group Instituto Gulbenkian de Ciência Rua da Quinta Grande, 6 2780-156 Oeiras Portugal Phone: +351 214464630 Fax: +351 214407970 Email: duquep@igc.gulbenkian.pt
