Enzyme-Linked Immunosorbent Assay (Elisa) by Marco Rito-Palomares
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Enzyme-Linked Immunosorbent Assay (Elisa)

Enzyme-Linked Immunosorbent Assay (Elisa)

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International Edition


About the Book

1 Fundamentals of ELISA: from the evolution of the immunoassays until invention of ELISAAbstract1. 1 Evolution of the immunoassays until invention of ELISA 1.1.1 Side chain theory1.1.2 Antigen-antibody binding theory1.1.3 Discovery of antibody structure1.1.4 Invention of RIA1.1.5 Invention and development of ELISA1.2 Principles of the immune system1.2.1 Antibody production in human body1.2.2 Categories of antibodies1.2.3 Antigen-antibody coupling1.2.4 Specificity of the antigen-antibody coupling1.3 Biomolecular interactions between antibody and antigen1.3.1 Hydrogen bonding1.3.2 Hydrophobic interaction1.3.3 Ionic attraction1.3.4 Van der Waals forces1.3.4.1 London dispersion1.3.4.2 Dipole-dipole interaction1.3.4.3 Ion-dipole forceReferences
2 General overview on applications of ELISAAbstract2.1 Application of ELISA2.1.1 Food industry2.1.2 Vaccine development2.1.3 Immunology2.1.3.1 Autoimmunity2.1.3.2 Humoral immunity2.1.4 Diagnosis2.1.4.1 Home pregnancy test2.1.4.2 Cancer detection2.1.4.3 Detection of the infectious diseases2.1.5 Toxicology 2.1.6 Drug monitoring and pharmaceutical industry2.1.7 TransplantationReferences
3 Step by step with ELISA: mechanism of operation, crucial elements, different protocols, and insights on immobilization and detection of various biomolecular entities Abstract3.1 Mechanism of operation3.2 Different elements of the assay3.2.1 Solid phase3.2.1.1 Adsorption3.2.2 Washing process3.2.3 Antigens 3.2.4 Antibodies3.2.4.1 Antispecies antibodies3.2.5 Enzyme3.2.5.2 Different types of enzyme3.2.5.2 Enzyme conjugation3.2.6 Substrate3.2.7 Stopping process3.2.8 Reading process3.2.8.1 Chromogenic assay3.2.8.2 Chemifluorescenct assay3.2.8.3 Chemiluminescent assay3.2.8.4 Reading apparatus3.2.9 Controls3.3 Target biomolecular entities by ELISA3.4 Different protocols3.4.1 Direct ELISA3.4.2 Indirect ELISA3.4.3 Sandwich ELISA3.4.4 Double sandwich ELISA3.4.5 Competitive ELISA3.5 Immobilization techniques for protein attachment 3.5.1 Physical immobilization3.5.2 Covalent immobilization3.5.2.1 Immobilization via zero-Length cross linker3.5.2.2 Immobilization via spacers3.5.3 Immobilization via entrapment3.5.4 Oriented immobilizationReferences
4 Evaluation of the detection results obtained from ELISAAbstract4.1 Conducting a reliable assay4.1.1 Sources of error4.1.2 Troubleshooting 4.2 Key parameters for evaluation of the assay4.2.1 Sensitivity4.2.2 Specificity4.2.3 Accuracy4.2.4 Limit of detection4.3 Measurable units in ELISAReferences
5 Advantages, disadvantages and modifications of conventional ELISA Abstract5.1 Significance of conventional ELISA 5.2 Shortages of conventional ELISA5.3 Materials of choice for fabrication of ELISA well plates5.4 Different types of ELISA well plates5.5 Modified ELISA platforms5.5.1 ELISpot5.5.2 Plasmonic ELISA5.5.3 Sphere-/bead-based ELISAs5.5.4 Paper-/fiber-based ELISAs5.5.
About the Author:

Dr. Samira Hosseini obtained her B.Sc. degree in Applied Physics, her M.Sc. degree in Polymer Chemistry and her PhD degree in Biomedical Engineering. Currently, she is working as a Postdoctoral Researcher at the School of Engineering and Sciences, Tecnológico de Monterrey, Mexico. She is the author/co-author of more than 15 ISI indexed scientific publications, 12 book chapters and the inventor/co-inventor of 4 Intellectual properties. She is a member of the Mexican National System of Researchers. Her main areas of research are surface engineering, interface science, bioanalytical assays and polymeric materials.

Dr. Patricia Vázquez-Villegas obtained her Bachelor's degree in Food Sciences Engineering program in 2008 and her PhD in Engineering Sciences from Tecnológico de Monterrey, Monterrey, México in 2013 majoring in Biotechnology. She made a one-year internship in 2012 at the University of British Columbia, Vancouver, Canada under the supervision of Dr. Charles A. Haynes. Since 2014 she holds a Postdoc position in Tecnológico de Monterrey where she is working in the development and application of platforms for purification and detection of different kinds of biomarkers. Dr. Vázquez is the author/co-author of 8 ISI indexed scientific publications, 1 book chapter and the inventor/co-inventor of 4 intellectual properties. She is a member of the Mexican National System of Researchers. Her main contribution had been in the field of continuous recovery of biomolecules using liquid-liquid extraction.

Professor Rito-Palomares obtained his PhD in Chemical Engineering from The University of Birmingham, United Kingdom in 1995. Following a sabbatical in the Department of Chemical Engineering at the University of Cambridge, United Kingdom (2001)., Prof. Rito-Palomares is currently the Director of the Biotechnology Centre and a Full Professor at Tecnologico de Monterrey, Mexico. He is a member of the Mexican Academy of Sciences, scientific committee of the International Foundation for Science (IFS, Sweden) as well as Mexican Research Council. He is the author/co-author of more than 100 ISI indexed scientific publications, 4 book chapters and inventor/co-inventor of 5 Intellectual properties that present his research work in the Bio-separation field. His main research contributions have been in the areas of biochemical recovery of biological products, and production, characterization and recovery of chemically modified proteins and the potential purification of stem cells.

Sergio O. Martinez is Professor of the Electrical and Computer Engineering Department at Tecnológico de Monterrey, Monterrey Campus; and researcher and chair of the Sensors and Devices area of the National School of Engineering and Sciences at the same Institute. Sergio O. Martínez received the BS degree in Electronics and Communications Engineering and the MS degree in Control Engineering both from Tecnológico de Monterrey, Campus Monterrey, Mexico in 1983 and 1985, respectively. He obtained a MS degree in Electronics from Philips International Institute, Eindhoven, Netherlands in 1990; and the PhD degree in Microelectronics from National Polytechnic Institute of Grenoble, France in 2002. Prof. Martínez-Chapa is a member of the Mexican National System of Researchers since 2004 as well as member of the Mexican Academy of Sciences since 2010. His recent research activities, focused on Micro/Nanotechnologies, include Microfabrication Processes for Particle Manipulation Devices, Electrokinetically driven Microdevices for Manipulation of Suspended Particles in Fluidic Media, and Modeling and Design of surface plasmon resonance (SPR)-based Biosensing Platforms. He has authored/co-authored 75 peer-reviewed publications, 7 book chapters and the inventor/co-inventor of 2 granted intellectual properties.


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Product Details
  • ISBN-13: 9789811067655
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Edition: 1st ed. 2018
  • Language: English
  • Returnable: Y
  • Sub Title: From A to Z
  • Width: 156 mm
  • ISBN-10: 9811067651
  • Publisher Date: 31 Jan 2018
  • Binding: Paperback
  • Height: 234 mm
  • No of Pages: 115
  • Spine Width: 7 mm
  • Weight: 240 gr


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