Erbb Receptor Signaling

Part I: Introduction and Review

1. ErbB Receptors and Cancer

Zhixiang Wang

2. New Insights from Drosophila into the Regulation of EGFR Signaling

Nicholas Harden

3. C. elegans Vulva Induction: An In Vivo Model to Study Epidermal Growth Factor Receptor Signaling and Trafficking

4. Targeting HER2 in Advanced Breast Cancer

Xiaofu Zhu and Anil Abraham Joy

Part II: Classical Methods in the Studies of ErbB Receptor Signaling

5. Methods to Investigate EGFR U

Alexia Conte and Sara Sigismund

6. Dimerization Assessment of Epithelial Growth Factor Family of Receptor Tyrosine Kinases by Using Cross-Linking Reagent

Hamid Maadi, Babak Nami, and Zhixiang Wang

7. Application of Immunofluorescence Staining to Study ErbB Family of Receptor Tyrosine Kinases

Babak N. Mollalou and Zhixiang Wang

8. Activation of Endosome-Associated Inert EGF Receptor Following Internalization

Yi Wang, Sukhmani Billing, and Zhixiang Wang

9. Two-Pulse Endosomal Stimulation of Receptor Tyrosine Kinases Induces Cell Proliferation

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10. Study of EGFR Signalling/Endocytosis by Site-Directed Mutagenesis

Qian Wang and Zhixiang Wang

11. Using Percoll Gradient Fractionation to Study the Endocytic Trafficking of the EGFR

Julie S. Gosney and Brian P. Ceresa

12. Analysis of Epidermal Growth Factor Receptor-Induced Cell Motility by Wound Healing Assay

Junfeng Tong and Zhixiang Wang

Part III: Recently Developed Methods in the Studies of ErbB Receptor Signaling

13. Cell Cycle Synchronization of HeLa Cells to Assay EGFR Pathway A

14. Analysis of Constitutive EGFR Signaling Regulating IRF3 Transcriptional Activity in Cancer Cells

Gao Guo, Ke Gong, and Amyn A. Habib

15. Measurement of Epidermal Growth Factor Receptor-Derived Signals Within Plasma Membrane Clathrin Structures

Stefanie Lucarelli, Ralph Christian Delos Santos, and Costin N. Antonescu

Part IV: Methods for Studying EGFR Signaling in Drosophila

16. Studying Non-Proliferative Roles for Egfr Signaling in Tissue Morphogenesis Using Dorsal Closure of the Drosophila Embryo

Bruce Reed and Nicholas H

Part V: Methods Related to the Translational Research of ErbB Receptor Signaling

17. Analysis of Epithelial-Mesenchymal Transition Induced by Overexpression of Twist

Jing-Wen Bai, Yong-Qu Zhang, Yao-Chen Li, and Guo-Jun Zhang

18. Assessment of Specificity of an Adenovirus Targeted to HER3/4

Sheena H. MacLeod, Kyle G. Potts, Shyambabu Chaurasiya, and Mary M. Hitt

19. Isolation of Human Mesenchymal Stem Cells for Studying ErbB Receptor Signaling

Chao Chen and Hongxing Jiang