1. An Introduction to Erythropoiesis Approaches
Joyce A. Lloyd
2. Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis
Martin D. Kafina and Barry H. Paw
3. Mouse Models of Erythropoiesis and Associated Diseases
Matthew P. Parker and Kenneth R. Peterson
4. Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells Skye C. McIver, Kyle J. Hewitt, Xin Gao, Charu Mehta, Jing Zhang, and Emery H. Bresnick
5. Stress Erythropoiesis Model Systems
&
nbsp; Laura F. Bennett, Chang Liao, and Robert F. Paulson
6. Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
Marie Trudel and Josepha-Clara Sedzro
7. Functional Analysis of Erythroid Progenitors by Colony-Forming Assays
James Palis and Anne Koniski
8. Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands
Jia Hao Yeo, Matthew P. Cosgriff, and Stuart T. Fraser
9. Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells
Xiuli An and Lixiang Chen
10. Analysis of Erythropoiesis Using Imaging Flow Cytometry
Theodosia Kalfa and Kathleen E. McGrath
11. Flow Cytometric Analysis of Erythroblast Enucleation
Xiuli An and Lixiang Chen
12. High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
Alyson Smith, Roberta B. Nowak, and Velia M. Fowler
13. Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
Ivan Krivega and Ann Dean
14. Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
Ivan Krivega and
Ann Dean
15. Genome Editing of Erythroid Cell Culture Model Systems
Jinfen J. Yik, Merlin Crossley, and Kate G.R. Quinlan
16. In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood
Anna Kovilakath, Safa Mohamad, Farrah Hermes, Shou Zhen Wang, Gordon D. Ginder, and Joyce A. Lloyd
17. Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines
Divya S. Vinjamur and Daniel E. Bauer
18. Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products
&nbs
p; Neil W.A. McGowan, John D.M. Campbell, and Joanne C. Mountford