About the Book
Preface...
Table of Contents...
Contributing Authors...
Part I Analysis of Ethylene Biosynthesis 1. Gas Chromatography-Based Ethylene Measurement of Arabidopsis Seedlings
Gyeong Mee Yoon and Yi-Chun Chen 2. Plant Ethylene Detection Using Laser Based Photo-Acoustic Spectroscopy
Bram Van de Poel and Dominique Van Der Straeten
3. Treatment of Plants with Gaseous Ethylene and Gaseous Inhibitors of Ethylene Action
Mark L. Tucker, Joonyup Kim, and Chi-Kuang Wen
4. Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast System
Won-Yong Song, Sumin Lee, and Moon-Soo Soh
5. Escherichia coli-Based Expression and In Vitro Activity Assay of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase and ACC Oxidase
Shigeru Satoh and Yusuke Kosugi
6. Assay Methods for ACS Activity and ACS Phosphorylation by MAP Kinases In Vitro and In Vivo
Xiaomin Han, Guojing Li, and Shuqun Zhang
Part II Analysis of the Ethylene Signaling Pathway
7. Analysis of Ethylene Receptors: Ethylene Binding Assays
Brad M. Binder and G. Eric Schaller
8. Analysis of Ethylene Receptors: Assay for Histidine Kinase Activity
G. Eric Schaller and Brad M. Binder
9. Analysis of Ethylene Receptor Interactions by Co-Immunoprecipitation Assays
Zhiyong Gao and G. Eric Schaller
10. Localization of the Ethylene-Receptor Signaling Complex to the Endoplasmic Reticulum: Analysis by Two-Phase Partitioning and Density Gradient Centrifugation
G. Eric Schaller
11. Kinase Assay for CONSTITUTIVE TRIPLE RESPONSE 1 (CTR1) in Arabidopsis thaliana
Han Yong Lee and Gyeong Mee Yoon
12. Circular Dichroism and Fluorescence Spectroscopy to Study Protein Structure and Protein-Protein Interactions in Ethylene Signaling
Mareike Kessenbrock and Georg Groth
Part III Analysis of Ethylene Responses
13. The Triple Response Assay and Its Use to Characterize Ethylene Mutants in Arabidopsis
Catharina Merchante and Anna N. Stepanova
14. Time-Lapse Imaging to Examine the Growth Kinetics of Arabidopsis Seedlings in Response to Ethylene
Brad M. Binder
15. Inhibitors of Ethylene Biosynthesis and Signaling
G. Eric Schaller and Brad M. Binder
16. Analysis of Growth and Molecular Responses to Ethylene in Etiolated Rice Seedlings
Biao Ma and Jin-Song Zhang
17. Love Me Not Meter: A Sensor Device for Detecting Petal Detachment Forces in Arabidopsis thaliana
Andrew Maule, Graham Henning, and Sara Patterson
18. Effects of Ethylene on Seed Germination of Halophyte Plants under Salt Stress