Part I. An Overview of Methods for Analyzing Vesicular Trafficking
1. Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets
Andrei I. Ivanov
2. Systematic Analysis of Endocytosis by Cellular Perturbations
Lena Kühling and Mario Schelhaas
Part II. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis
3. Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System
Oleg Varlamov
4. Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides
Guoqiang Xu, Alessia Deglincerti, Jeremy S. Paige, and Samie R. Jaffrey
5. Secretion of Circular Proteins Using Sortase
Karin Strijbis and Hidde Ploegh
6. Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation
Xuhang Li and Mark Donowitz
7. Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis
Katrin Eichelbaum and Jeroen Krijgsveld
Part III. Imaging and Ultrastructural Analysis of Vesicle Trafficking in Cultured Cells
8. Probabilistic Density Maps to Study the Spatial Organization of Endocytosis
Jean-Philippe Grossier, Bruno Goud, and Kristine Schauer
9. Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors
Antje Schmidt, Burkhard Wiesner, Ralf Schulein, and Anke Teichmann
10. Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells
Marc Fransen
11. SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells
Emily H. Stoops, Glen A. Farr, Michael Hull, and Michael J. Caplan
12. FLAsH-PALM: Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling
Mickael Lelek, Francesca Di Nunzio, and Christophe Zimmer
13. Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers
Anton Khmelinskii and Michael Knop
14. Synchronization of Secretory Cargo Trafficking in Populations of Cells
Gaelle Boncompain and Franck Perez
15. Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins
Yoshimi Haga and Tadashi Suzuki
16. A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering
Toru Komatsu and Takanari Inoue
17. A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays
Kazumasa Ohashi and Kensaku Mizuno
18. Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy
Daniel R. Passmore, Tejeshwar Rao, and Arun Anantharam
19. Nanocones to Study Initial Steps of Endocytosis
Sangmoo Jeong and Milos Galic
20. A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography
Nuria Jimenez and Jan A. Post
21. VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy
Matthia A. Karreman and Elly G. Van Donselaar
Part IV. Analysis of Exocytosis and Endocytosis in Model Organisms
22. Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network
Cecilia Rodriguez-Furlán, Glenn R. Hicks, and Lorena Norambuena
23. Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans
Jachen A. Solinger, Dmitry Poteryaev, and Anne Spang
24. Visualization of Clathrin-Mediated Endocytosis in Live Drosoph
