1. The Complex Interplay between Extracellular Matrix and Cells in Tissues
Dimitra Manou, Ilaria Caon, Panagiotis Bouris, Irene‐Eva Triantaphyllidou, Cristina Giaroni, Alberto Passi, Nikos K. Karamanos, Davide Vigetti, and Achilleas D. Theocharis
2. Method for Studying ECM Expression: In Situ RT-PCR
Elena Caravà, Cristiana Marcozzi, Barbara Bartolini, Marcella Reguzzoni, Paola Moretto, Ilaria Caon, Evgenia Karousou, Alberto Passi, and Manuela Viola
3. Visualizing the Supramolecular Assembly of Collagen
Mario Raspanti, Marcella Reguzzoni, Petra Rita Basso, Marina Protasoni, and Désirée Martini
4. Steady-state and Pulse-chase Analyses of Fibrillar Collagen
Antonella Forlino, Francesca Tonelli, and Roberta Besio
5. Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates
Mariana P. Stelling, Ananda de A. Bento, Philippe Caloba, Eduardo Vilanova, and Mauro S. G. Pavão
6. Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems
Chiara Paganini, Rossella Costantini, and Antonio Rossi
7. Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples Sanna Oikari and Markku I Tammi
8. Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis
Mary K. Cowman
9. Hyaluronan Pericellular Matrix: Particle Exclusion Assay
Melanie A. Simpson 10. Determination of Cell Surface Hyaluronan through Flow Cytometry
Daiana L. Vitale, Fiorella M. Spinelli, and Laura Alaniz
11. Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine
Michela Bistoletti, Paola Moretto, and Cristina Giaroni
12. Analyzing Hyaluronidases in Biological Fluids
Christos Velesiotis, Stella Vasileiou, and Demitrios H. Vynios
13. Method for Studying the Physical Effect of Extracellular Matrix on Voltage-dependent Ion Channels Gating Eleonora Solari and Andrea Moriondo
14. Methods for Monitoring Matrix-Induced Autophagy Carolyn Chen, Aastha Kapoor, and Renato V. Iozzo
15. Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography
Elin Hadler-Olsen and Jan-Olof Winberg
16. Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography
Elin Hadler-Olsen and Jan-Olof Winberg
17. In Vitro Spheroid Sprouting Assay of Angiogenesis
Fatema Tuz Zahra, Efrossini Choleva, Md Sanaullah Sajib, Evangelia Papadimitriou, and Constantinos Μ. Mikelis
18. Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis
Pinelopi Kastana, Fatema Tuz Zahra, Despoina Ntenekou, Stamatiki Katraki-Pavlou, Dimitris Beis, Michail S. Lionakis, Constantinos Μ. Mikelis, and Evangelia Papadimitriou
19. Exosomes from Cell Culture Conditioned Medium: Isolation by Ultracentrifugation and Characterization
Anurag Purushothaman
20. Preparation and Characterisation of Rich in Extracellular Matrix Tissue Surrogates Using the Principles of Macromolecular Crowding
Adrian Djalali-Cuevas, Sergio Garnica-Galvez, Andrea Rampin, Diana Gaspar, Ioannis Skoufos, Athina Tzora, Nikitas Prassinos, Nikolaos Diakakis, and Dimitrios I. Zeugolis
21. Novel Approaches for Extracellular Matrix Targeting in Disease Treatment
Nikolaos A. Afratis and Irit Sagi
