1. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine Mingyu Shang, John Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang
2. Red Blood Cells: A Source of Extracellular Vesicles
Winston Patrick Kuo, John Tigges, Vasilis Toxavidis, and Ionita Ghiran
Part I: Isolation of Extracellular Vesicles
3. Isolation of Extracelullar Vesicles by Ultracentrifugation
Fatemeh Momen-Heravi
4. Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles
Mitja L. Heinemann and Jody Vykoukal
5. Paper-Based Devices for Isolation of Extracellular Vesicles
Yi-Hsing Hsiao and Chihchen Chen
6. Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis Cindy M. Yamamoto, Taku Murakami, and Shu-Wing Ng
7. Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads
Ketil W. Pedersen, Bente Kierulf, and Axl Neurauter
Part II: Purification of Extracellular Vesicles
8. Polymer-Based Purification of Extracellular Vesicles
Peter N. Brown and Hang Yin
9. Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification
Richard Lobb and Andreas Möller
10. Purification Protocols for Extracellular Vesicles
Rebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill
Part III: Characterization of Extracellular Vesicles
11. Characterization of Extracellular Vesicles by Surface Plasmon Resonance
Hyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro
12. Extracellular Vesicle Isolation and Analysis by Western Blotting
Emma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church
13. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis
Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic
14. Characterization of Extracellular Vesicles by Flow Cytometry
Virginia Camacho, Vasilis Toxavidis, and John C. Tigges
15. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC) Tao Huang and Jiang He
16. Multi-Surface Antigen Staining of Larger Extracellular Vesicles
Veronika Lukacs-Kornek, Henrike Julich, Sabine Katharina Urban, and Miroslaw Kornek
17. Microcapillary Chip-Based Extracellular Vesicle Profiling System
Takanori Akagi and Takanori Ichiki
Part IV: Imaging of Extracellular Vesicles
18. Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy
Petr Cizmar and Yuana Yuana
19. Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy
Dmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci
Part V: Tracking of Extracellular Vesicles
20. In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase
Yuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura