Part I: EXPRESS DETECTION (from 3 minutes to 1 hour) 1. Express FRET Labeling and Analysis of Phagocytic Clearance
Vladimir V. Didenko
2. Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell Cycle Alterations
Nevenka Bihari
3. Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota 4. Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo
Hadi Tadayyon, Mehrdad J. Gangeh, Roxana Vlad, Michael C. Kolios, and Gregory J. Czarnota
5. Fluorochrome-Labeled Inhibitors of Caspases: Expedient In Vitro and In Vivo Markers of Apoptotic Cells for Rapid Cytometric Analysis
Zbigniew Darzynkiewicz, Hong Zhao, H. Dorota Halicka, Piotr Pozarowski, and Brian Lee
6. The Fast-halo Assay for the Detection of DNA Aamage
Piero Sestili, Cinzia Calcabrini, Anna Rita Diaz, Carmela Fimognari, and Vilberto Stocchi
7. Rapid Detection of Bacterial Susceptibility or Resistance to Quinolones
Fátima Otero, Rebeca Santiso, María Tamayo, Germán Bou, Jaime Gosálvez, and José Luis Fernández
8. Rapid Detection of Apoptosis in Cultured Mammalian Cells
Kudryavtsev I., Serebryakova M., Solovjeva L., Svetlova M., and Firsanov D 9 Quick Detection of DNase II-type Breaks in Formalin-Fixed Tissue Sections
Candace. L. Minchew and Vladimir V. Didenko
Part II: RAPID DETECTION (3 hours or less)
10. Express γ-H2AX Immunocytochemical Detection of DNA Damage
Nate Hopp, Jodi Hagen, Birte Aggeler, and Alexander E. Kalyuzhny
11. Rapid detection of γ-H2AX by Flow Cytometry in Cultured Mammalian Cells Firsanov D., Solovjeva L., Lublinskaya O., Zenin V., Kudryavtsev I., Serebryakova M., and Svetlova M.
12. Rapid Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image- Cytometry
Hong Zhao and Zbigniew Darzynkiewicz 13. Fast Micromethod: Determination of DNA Integrity in Cell Suspensions and in Solid Tissues
Nevenka Bihari
14. Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR
Nadja Patenge
15. Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt Ended DNA Breaks by In Situ Ligation
Vladimir V. Didenko
Part III: ACCELERATED DETECTION (time-saving versions of conventional techniques) 16. Twelve Gel Comet Assay Format for Quick Examination of DNA Damage and Repair
Sergey Shaposhnikov and Andrew Collins
17. Immunofluorescence Analysis of γ-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle
Solovjeva L., Firsanov D., Pleskach N., and Svetlova M.
18. RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA
Rosanna Tofalo and Aldo Corsetti
19. Rapid Detection of γ-H2Av Foci in ex vivo MMS-treated Drosophila Imaginal Discs
Varandt Y. Kh
