Part I Basic Fluorescence Imaging
1 Fluorescence Microscopy: A Field Guide for Biologists.
Lucy Swift and Pina Colarusso
2 Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy.
Richard Manivanh, Seema S. Lakdawala, and Jennifer E. Jones
3 Optimizing Long-Term Live Cell Imaging. Alex Lac, Austin Le Lam, and Bryan Heit
Part II Quantitative Approaches
4 Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy.
Janhavi Nagwekar, Caterina Di Ciano-Oliveira, and Gregory D. Fairn
5 Bimolecular Fluorescence Complementation to Visualize Protein: Protein Interactions in Cells.
Cassandra R. Edgar and Jimmy D. Dikeakos
6 Monitoring Cellular Responses to Infection with Fluorescent Biosensors.
Amena Aktar, Kasia M Wodz, and Bryan Heit
7 Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF).
Erika Jang, Siavash Ghaffari, and Warren L. Lee
8 Measurement of Minute Cellular Forces by Traction Force Microscopy.
Valentin Jaumouillé
Part III Tissue and Live Animal Imaging
9 Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues.
Lane B. Buchanan, Zhongtian Shao, Yuan Chung Jiang, Abbie Lai, Thomas J. Hope, Ann M. Carias, and Jessica L. Prodger
10 Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin.
Madison Turk, Jeff Biernaskie, Douglas J. Mahoney, and Craig N. Jenne
11 Quantifiable Intravital Light Sheet Microscopy.
Holly C. Gibbs, Sreeja Sarasamma, Oscar R. Benavides, David G. Green, Nathan A. Hart, Alvin T. Yeh, Kristen C. Maitland, and Arne C. Lekven
12 Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing.
Frank L. Jalufka, Sun Won Min, Madison E. Platt, Anna L. Pritchard, Theodore E. Margo, Alexandra O. Vernino, Megan A. Kirchhoff, Ryan T. Massopust, and Dylan A. McCreedy
13 Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.
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