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Fluorescent Protein-Based Biosensors

Fluorescent Protein-Based Biosensors


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About the Book

1. The Design and Application of Genetically-Encodable Biosensors Based on Fluorescent Proteins

Robert H. Newman and Jin Zhang

2. An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications

J. Goedhart, Mark A. Hink, and Kees Jalink

3. Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells using Genetically Encoded Fluorescent Biosensors

J. Genevieve Park and Amy E. Palmer

4. Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells

J. Klarenbeek and Kees Jalink

5. Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters

Alessandra Stangherlin, Andreas Koschinski, Anna Terrin, Anna Zoccarato, He Jiang, Laura Ashley Fields, and Manuela Zaccolo

6. Genetically Encoded Fluorescent Biosensors for Live-cell Imaging of Lipid Dynamics

Moritoshi Sato

7. Live Cell Imaging of Cytosolic NADH-NAD+ Redox State using a Genetically Encoded Fluorescent Biosensor

Yin Pun Hung and Gary Yellen

8. Measuring Membrane Voltage with Microbial Rhodopsins

Daniel R. Hochbaum and Adam Cohen

9. Imaging the Activity of Ras superfamily GTPase Proteins in Small Subcellular Compartments in Neurons

Ana F. Oliveira and Ryohei Yasuda

10. Imaging Kinase Activity at Protein Scaffolds

Maya T. Kunkel and Alexandra C. Newton

11. Using a Genetically Encoded FRET-Based Reporter to Visualize calcineurin phosphatase Activity in Living Cells

Sohum Meta and Jin Zhang

12. Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation

Kazuki Sasaki and Minoru Yoshida

13. Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity

Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang

14. Biosensor Imaging in Brain Slice Preparations

Marina Polito, Pierre Vincent, and Elvire Guiot

15. Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster

Shubham Dipt, Thomas Riemensperger, and André Fiala

16. Multi-Parameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel

Nwe-Nwe Aye-Han and Jin Zhang

17. FRET and BRET-Based Biosensors in Live Cell Compound Screens

Katie Herbst-Robinson, Jessica Yang, and Jin Zhang

18. Integrating Fluorescent Biosensor Data Using Computational Models

Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffery J. Saucerman

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Product Details
  • ISBN-13: 9781627036214
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Depth: 19
  • Height: 261 mm
  • No of Pages: 251
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 184 mm
  • ISBN-10: 1627036210
  • Publisher Date: 20 Sep 2013
  • Binding: Hardback
  • Edition: 2014 ed.
  • Language: English
  • Returnable: Y
  • Spine Width: 21 mm
  • Weight: 725 gr

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