1. Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research
Catherine M. With, David L. Evers, and Jeffrey T. Mason
2. Tissue Microarrays: Construction and Uses
Carol B. Fowler, Yan-Gao Man, Shimin Zhang, Timothy J. O'Leary, Jeffrey T. Mason, and Robert E. Cunningham
3. Standardization in Immunohistology
Anthony S-Y Leong and Trishe Y-M Leong
4. Multiple Immunofluorescence Labeling of Formalin-fixed Paraffin-embedded (FFPE) Tissue
David Robertson and Clare M. Isacke
5. Microwaves for Chromogenic-in-situ-hybridization
Anthony S-Y Leong and Zenobia Haffajee
6. Automated Analysis of FISH-stained HER2/neu Samples with Metafer
Christian Schunck and Eiman Mohammad
7. Laser Capture Microdissection (LCM) of FFPE Tissue Sections
Bridging the Gap between Microscopy and Molecular Analysis
Renate Burgemeister
8. Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections
Renate Burgemeister
9. Microarray-based CGH and Copy Number Analysis of FFPE Samples
Fahd Al-Mulla
10. Microarray Profiling of DNA Extracted from FFPE Tissues using SNP6.0 Affymetrix Platform
Marianne Tuefferd, An de Bondt, Ilse Van den Wyngaert, Willem Talloen, and Hinrich Göhlmann
11. Whole Genome Amplification of DNA Extracted From FFPE Tissues
Mira Bosso and Fahd Al-Mulla
12. Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-embedded Tissue
Brendan Doyle, Ciarán O'Riain, and Kim Appleton
13. Analysis of DNA Methylation in FFPE Tissues using the MethyLight Technology
Ashraf Dallol, Waleed Al- Ali, Amina Al-Shaibani, and Fahd Al-Mulla
14. RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions
Mei-Lan Liu, Jennie Jeong, Ranjana Ambannavar, Carl Millward, Frederick Baehner, Chithra Sangli, Debjani Dutta, Mylan Pho, Anhthu Nguyen, and Maureen T. Cronin
15. RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed Paraffin Embedded Tissues
Aaron Scott, Ranjana Ambannavar, Jennie Jeong, Mei-Lan Liu and Maureen T. Cronin
16. microRNA Isolation from Formalin-Fixed Paraffin-Embedded Tissues
Aihua Liu and Xiaowei Xu
17. Gene Expression Profiling of RNA Extracted from FFPE tissues: NuGEN Technologies' Whole-Transcriptome Amplification System
Leah Turner, Joe Don Heath, and Nurith Kurn
18. Protein Mass Spectrometry Applications on FFPE Tissue Sections
Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason
19. An Alternative Fixative to Formalin Fixation for Molecular Applications: the RCL2â-CS100 Approach
Amélie Denouël, Florence Boissière-Michot, Philippe Rochaix, Frédéric Bibeau, Nathalie Boulle
