Foxo Transcription Factors

1. Introduction to FOXO Biology

Wolfgang Link

Part I: Biochemical and Molecular Methods

2. Characterization of microRNAs Regulating FOXO Expression

3. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter

Christopher P. Cardozo

4. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover

Thomas Heimbucher and Coleen T. Murphy

5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity

Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco

6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes

7. Characterization of FOXO Acetylation

Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam

8. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays

Antonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida

9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes

Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira

10. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters

11. RNAi Mediated Silencing of FoxO Factors

Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas

Part II: Imaging Approaches

12. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO

Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain

13. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO

Marta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos

14. Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus

Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link

15. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology

Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato

Part III: Omics and Bioinformatic Approaches

16. Genome-wide Analysis for Identifying FOXO Protein Binding Sites

Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang

Bradford E. Peercy and Martin F. Schneider

18. Following Transcriptome to Uncover FOXO Biological Functions

Raymond Liang, Vijay Menon, and Saghi Ghaffari

Part IV: Animal Models

19. Hydra as Model to Determine the Role of FoxO in Longevity

Thomas C. G. Bosch

Hwanhee Oh and Jihye Paik

Part V: Human Gentic and Clinical Studies

21. Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity

Timothy A Donlon, Philip MC Davy, and Bradley J Willcox

22. Analysis of FOXO3a Gene Polymorphism Associated with Asthma