Preface
Contributors
I. Introduction
1. Fungal Transformation: From Protoplasts to Targeted Recombination Systems
Juan F. Martín
II. Transformation Methods: Protoplast Transformation
2. Protoplast Transformation for Genome Manipulation in Fungi
Aroa Rodriguez-Iglesias and Monika Schmoll
3. Trichoderma Transformation Methods
Mónica G. Malmierca, Rosa E. Cardoza and Santiago Gutiérrez
4. Transformation of Mucor circinelloides f. lusitanicus Protoplasts
Victoriano Garre, José Luis Barredo and Enrique A. Iturriaga
5. Transformation of Saccharomyces cerevisiae: Spheroplast Method
Shigeyuki Kawai and Kousaku Murata
III. Transformation Methods: Electroporation
6. Electroporation Mediated DNA Transformation of Filamentous Fungi
B.N. Chakraborty
7. Chemical Transformation of Candida albicans
Sophie Bachellier-Bassi and Christophe d'Enfert
8. Electroporation of Pichia pastoris
Knut Madden, Ilya Tolstorukov and James Cregg
9. Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli)
Kostyantyn Dmytruk and Andriy Sibirny
IV. Transformation Methods: Particle Bombardment
10. Biolistic Transformation for Delivering DNA into the Mitochondria
Arianna Montanari, Monique Bolotin-Fukuhara, Mario Fazzi D'Orsi, Cristina De Luca, Michele M. Bianchi and Silvia Francisci 11. Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants
Jingwen Zhou, Liming Liu, Guocheng Du and Jian Chen
12. Use of the Biolistic Particle Delivery System to Transform Fungal Genomes
V. S. Junior Te'o and K. M. Helena Nevalainen
13. Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment
Eiji Sakuradani, Hiroshi Kikukawa, Seiki Takeno, Akinori Ando, Sakayu Shimizu and Jun Ogawa
V. Transformation Methods: Agrobacterium-Mediated Transformation
14. Agrobacterium tumefaciens-Mediated Transformation
Rasmus John Normand Frandsen
15. Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts
Giuseppe Ianiri and Alexander Idnurm
16. Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum
Manish Pareek, Mahak Sachdev, Meenakshi Tetorya and Manchikatla V. Rajam
VI. Transformation Methods: Li-acetate Transformation
17. High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method
R. Daniel Gietz
18. Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism
Shigeyuki Kawai and Kousaku Murata
19. Transformation of Lithium Acetate-treated Neurospora crassa
John V. Paietta
VII. Transformation Methods: New developments
20. Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells
Yevhen Filyak, Nataliya Finiuk, Nataliya Mitina, Alexander Zaichenko and Rostyslav Stoika
21. Transformation of Fungi Using Shock Waves
Miguel A. Gómez-Lim, Denis Magaña Ortíz, Francisco Fernández and Achim M. Loske
VIII. Exogenous DNA: Uptake of DNA
22. Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics
Petar Tomev Mitrikeski
23. Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle
Aurelijus Zimkus, Audrius Misiūnas, Arūnas Ramanavičius and Larisa Chaustova
IX. Exogenous DNA: Integration of DNA
24.
About the Author:
Marco A. van den Berg, PhD
Principal Scientist Applied Biochemistry and Screening
DSM Biotechnology Center
Delft, The Netherlands
Karunakaran Maruthachalam, PhD
Current: Research Scientist
Global Marker Technology Lab, DuPont-Pioneer
DuPont Knowledge Center
Andhra Pradesh, India
Previous: Molecular Biologist
California Seed and Plant Labs
Pleasant Grove, CA, USA
