About the Book
Part I: Setting and Performing ELISPOT Assay
1. Unique Strengths of ELISPOT for T Cell Diagnostics
Paul V. Lehmann and Wenji Zhang
2. The Impact of Harmonization on ELISPOT Assay Performance
7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion
Jun-Seop Shin and Chung-Gyu Park
10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
Part IV. Image and Data Analysis
11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity
Sylvia Janetzki and Cedrik M. Britten
Part II: ELISPOT for Veterinary Research
3. Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells
7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion
Jun-Seop Shin and Chung-Gyu Park
10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
Part IV. Image and Data Analysis
11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity
Jodi Hagen, Chris Hartnett, J.P. Houchins, Steeve Giguère, and Alexander E. Kalyuzhny
4. Utilization of Feline ELISPOT for Mapping Vaccine Epitopes
7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
9. ELISPOT Assay as a Tool to Study the Effects of Stem Cells on Cytokine Secretion
Jun-Seop Shin and Chung-Gyu Park
10. Combining ELISPOT and ELISA to Measure Amounts of Cytokines Secreted by a Single Cell
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
Part IV. Image and Data Analysis
11. How ELISPOT Morphology Reflects on the Productivity and Kinetics of Cells' Secretory Activity
Jeffrey R. Abbott, Ruiyu Pu, James K. Coleman, and Janet K. Yamamoto
5. Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay
7. ELISPOT Assay as a Tool to Study Oxidative Stress in Lymphocytes
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
8. ELISPOT Assay for Neuroscience Research: Studying TNFa Secretion from Microglial Cells
Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny
9.