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Hepatitis C Virus Protocols

Hepatitis C Virus Protocols


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About the Book

Part I: Review of Hepatitis C Virus and Bioinformatic Tools

1. Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus

Darrick K. Li and Raymond T. Chung

2. Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV

Laura Riva and Jean Dubuisson

3. Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR)

Yun Zhang, Christian Zmasek, Guangyu Sun, Christopher N. Larsen, and Richard H. Scheuermann

Part II: Methods for Virus Cloning, Culture, and Purification

4. Establishment of Replication-competent HCV Strain with Minimum Modifications

Takanobu Kato, Asako Murayama, and Takaji Wakita

5. Full-length Open Reading Frame Amplification of Hepatitis C Virus

Ulrik Fahnøe and Jens Bukh

6. InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones

Barnabas King, Richard Urbanowicz, Alexander W. Tarr, Jonathan K. Ball, and C. Patrick McClure

7. Production and Purification of Cell Culture Hepatitis C Virus

Cynthia de la Fuente and Maria Teresa Catanese

8. Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture

Xianfang Wu and Viet Loan Dao Thi

Part III: Methods for the Study of HCV Life Cycle

9. Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing

Hui Li, Muhammad N. Zahid, Shuyi Wang, and George M. Shaw

10. A Cell Culture Model for Persistent HCV Infection

Victoria Castro, Ginés Ávila, Lidia Mingorance, and Pablo Gastaminza

11. Expression Cloning of Host Factors Required for the HCV Replication Cycle

Sandra Ciesek and Thomas von Hahn

12. Application of Deep Mutational Scanning in Hepatitis C Virus

Nicholas C. Wu and Hangfei Qi

13. Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals

Shira Perez and Meital Gal-Tanamy

14. Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay

Allison E. Roder and Stacy M. Horner

15. Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells

Solène Denolly, François-Loïc Cosset, and Natalia Freitas

16. Functional Characterization of Apolipoproteins in the HCV Life Cycle

Luhua Qiao and Guangxiang (George) Luo

17. Investigating Hepatitis C Virus Infection Using Super-resolution Microscopy

Pedro Matos Pereira, Caron Jacobs, and Joe Grove

18. Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes

Yasmine Baktash and Glenn Randall

19. Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins

Richard A. Urbanowicz, Jonathan K. Ball, and Alexander W. Tarr

20. Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis

Fang Chen, Erick Giang, Radhika Gopal, and Mansun Law

21. Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells

Samantha A. Yost, Jillian Whidby, Abdul G. Khan, Yuanyuan Wang, and Joseph Marcotrigiano

Part IV: Methods for Studying Immune Responses

22. Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact

Séverin Coléon, Sonia Assil, and Marlène Dreux


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Product Details
  • ISBN-13: 9781493989751
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Height: 254 mm
  • No of Pages: 508
  • Series Title: Methods in Molecular Biology
  • Weight: 1178 gr
  • ISBN-10: 1493989758
  • Publisher Date: 28 Dec 2018
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Spine Width: 29 mm
  • Width: 178 mm


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