Preface...
Table of Contents...
Contributing Authors...
1. Strategies and Considerations for Improving Expression of 'Difficult to Express' Proteins in CHO Cells
Christina S. Alves and Terrence M. Dobrowsky
2. Glycoengineering of CHO Cells to Improve Product Quality
Qiong Wang, Bojiao Yin, Cheng-Yu Chung, and Michael J. Betenbaugh
3. Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension
Yashas Rajendra, Sowmya Balasubramanian, and David L. Hacker
4. Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression
Patrick Mayrhofer and Renate Kunert
5. Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells
Eric Baek, Soo Min Noh, and Gyun Min Lee
6. Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors
Alan Costello, Nga Lao, Martin Clynes, and Niall Barron
7. Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
Lise Marie Grav, Karen Julie la Cour Karottki, Jae Seong Lee, and Helene Faustrup Kildegaard
8. Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture
Zeynep Betts and Alan J. Dickson
9. Selection of High-Producing Clones using FACS for CHO Cell Line Development
Clair Gallagher and Paul S. Kelly
10. The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
Hussain Dahodwala and Susan T. Sharfstein
11. A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data
Craig Monger, Krishna Motheramgari, John McSharry, Niall Barron, and Colin Clarke
12. Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells
Orla Coleman, Michael Henry, Martin Clynes, and Paula Meleady
13. Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells
Michael Henry, Orla Coleman, Prashant, Martin Clynes, and Paula Meleady
14. Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture
Yuzhou Fan, Helene Faustrup Kildegaard, and Mikael Rørdam Andersen
15. Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells
Sara Carillo, Stefan Mittermayr, Amy Farrell, Simone Albrecht, and Jonathan Bones
16. Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses
Catherine E.M. Hogwood, Lesley M. Chiverton, and C. Mark Smales
