Part I: Reagents and Kits for Measuring Cells and their Phenotypes 1. Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays
Roy Edward
2. General Staining and Segmentation Procedures for High Content Imaging and Analysis
Kevin M Chambers, Bhaskar S Mandavilli, Nick J Dolman and Michael S Janes
3. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis Bhaskar S Mandavilli, Robert J Aggeler, and Kevin Chambers
4. Cell-based High Content Analysis of Cell Proliferation and Apoptosis
Bhaskar S Mandavilli, Michelle Yann and Scott Clarke
5. Tools to Measure Autophagy using High Content Imaging and Analysis
Nick J Dolman, Brent A. Samson, Kevin M. Chambers, Michael S Janes, and Bhaskar S Mandavilli
Part II: Selecting Microtiter Plates, Identify Imaging Artifacts, Rnai Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS
6. Guidelines for Microplate Selection in High Content Imaging
O.J. Trask
7. Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler
Mark-Anthony Bray and Anne E. Carpenter
8. High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening
Steven A. Haney
9. Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays
Karol Kozak, Bernt Rinn, Oliver Leven, and M. Emmenlauer
10. Live-Cell High Content Screening in Drug Development
Milan Esner, Felix Meyenhofer, and Marc Bickle
11. Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening
Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O'Connell, Martyn Banks, and Andrea Weston
Part III: Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis
12. Translocation Biosensors - Versatile Tools To Probe Protein Functions In Living Cells
Verena Fetz, Roland H. Stauber, and Shirley K. Knauer
13. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions
Yun Hua, Daniel P. Camarco, Christopher J. Strock, and Paul A. Johnston
14. High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines
Paul A. Johnston, Malabika Sen, Yun Hua, Daniel Camarco, Tong Ying Shun, John S. Lazo, and Jennifer R. Grandis
15. Single Cell and Population Level Analysis of HCA Data
David Novo, Kaya Ghosh, and Sean Burke
16. Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens
Judith Wardwell-Swanson and Yanhua Hu
Section IV: Phenotypic Models - Primary Neurons, Neural Stem/Progenitor Cells, Induced-Pluripotent Stem Cells, Multicellular Tumor Spheroids, Endothelial/Mesenchymal Stem Cell Co-Cultures, and Zebrafish Embryos
17. High Content Screening of Mammalian Primary Cortical Neurons
Dario Motti, Murray Blackmore, John Bixby, and Vance Lemmon
18. Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications
Joshua A. Harrill
19. Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays Oksana Sirenko and Evan F. Cromwell
20. The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates
David A. Close
