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High-Throughput Screening Assays in Toxicology

High-Throughput Screening Assays in Toxicology


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About the Book

Part I: In Vitro Toxicological High-Thoughput Screening Methods

1. Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay

Carlos Coriano, Emily Powell, and Wei Xu

2. Mitochondrial Membrane Potential Assay

Srilatha Sakamuru, Matias S. Attene-Ramos, and Menghang Xia

3. Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway

Thai Khuc, Chia-Wen (Amy) Hsu, Srilatha Sakamuru, and Menghang Xia

4. Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators

Caitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia

5. Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity

Chia-Wen (Amy) Hsu, Jinghua Zhao, and Menghang Xia

6. Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway

Jinghua Zhao, Sunita J. Shukla, and Menghang Xia

7. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells

Yitong Liu

8. Determination of Histone H2AX Phosphorylation in DT40 Cells

Kana Nishihara, Sampada A. Shahane, and Menghang Xia

9. High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells

Sampada A. Shahane, Kana Nishihara, and Menghang Xia

Part II: In Vivo Toxicological High-Thoughput Screening Methods

10. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish

Lisa Truong, Michael T. Simonich, and Robert L. Tanguay

11. Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans

Zachary Lundby, Jessica Camacho, and Patrick Allard

Part III: In Silico High-Thoughput Screening Toxcity Data Analysis

12. A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling

Ruili Huang

13. Correction of Microplate Data from High-Throughput Screening

Yuhong Wang and Ruili Huang

14. CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints

Alexander Sedykh

15. Accounting Artifacts in High-Throughput Toxicity Assays

Jui-Hua Hsieh

16. Accessing the High-Throughput Screening Data Landscape

Daniel P. Russo and Hao Zhu

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Product Details
  • ISBN-13: 9781493963447
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: 1st ed. 2016
  • Language: English
  • Returnable: Y
  • Spine Width: 13 mm
  • Width: 178 mm
  • ISBN-10: 1493963449
  • Publisher Date: 13 Aug 2016
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 175
  • Series Title: Methods in Molecular Biology
  • Weight: 598 gr

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