Part I: The Hippo Pathway: Drosophila Genetics Lead the Way
1. The Power of Drosophila Genetics: The Discovery of the Hippo Pathway
Rewatee Gokhale and Cathie M. Pfleger
2. Drosophila Genetics: The Power of Genetic Mosaic Approaches
Mardelle Atkins
3. Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues
Alexander D. Fulford and Paulo S. Ribeiro
4. Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs
Jiajie Xu, Ting Su, Sherzod A. Tokamov, and Richard G. Fehon
5. Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence
Cordelia Rauskolb and Kenneth D. Irvine
6. Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions
Yurika Matsui and Zhi-Chun Lai
Part II: Molecular and Cell Biology Studies of the Hippo Pathway
7. Immunohistochemistry to Study YAP in Human Tissue Samples
Franziska Haderk, Victor Olivas, and Trever G. Bivona
8. Immunofluorescence Study of Endogenous YAP in Mammalian Cells
Valentina Rausch and Carsten G. Hansen
9. Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells
Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet, and Xaralabos Varelas
10. Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors
Maria Chatzifrangkeskou and Eric O'Neill
11. Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells
Sirio Dupont
12. Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells
Xiaolei Cao and Bin Zhao
13. HTRF(R) Total and Phospho-YAP(Ser127) Cellular Assays
Diana Zindel, Claire Vol, Odile Lecha, Isabelle Bequignon, Merve Bilgic, Marion Vereecke, Fabienne Charrier-Savournin, Maïté Romier, Eric Trinquet, Jean-Philippe Pin, Julie Pannequin, Thomas Roux, Elodie Dupuis, and Laurent Prézeau
14. Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids
Yoichi Asaoka, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp Heisenberg, and Makoto Furutani-Seiki
15. Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview
Sirio Dupont
16. CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels
Ryan J. Quinton and Neil J. Ganem
17. Hippo Pathway Reg