1. In Vitro Assembly of Nucleosomes For Binding/Remodeling Assays
Arnob Dutta and Jerry L. Workman
2. An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro
Liette Laflamme, Benoit Guillemette, and Luc Gaudreau
3. Purification of Yeast Native Reagents for the Analysis of Chromatin Function-I: Nucleosomes for Reconstitution and Manipulation of Histone Marks
Nicolas Lacoste, Wajid Bhat, and Jacques Côté
4. Purification of Yeast Native Reagents for the Analysis of Chromatin Function-II: Multiprotein Complexes and Biochemical Assays
Nicolas Lacoste, Wajid Bhat, and Jacques Côté
5. Histone Purification from Saccharomyces Cerevisiae
Frederic Jourquin and Vincent Géli
6. Analytical Ultracentrifuge Analysis of Nucleosomes Assembled from Recombinant, Acid Extracted, HPLC-Purified Histones
Manjinder S. Cheema and Juan Ausió
7. Silac-Based Quantitative Strategies for Accurate Histone Post-Translational Modifications Profiling Across Multiple Biological Samples
Alessandro Cuomo, Monica Soldi, and Tiziana Bonaldi
8. Characterization of Individual Histone Post-Translational Modifications and their Combinatorial Patterns by Mass Spectrometry-Based Proteomics Strategies
Simone Sidoli and Benjamin A. Garcia
9. Production and Purification of Antibodies against Histone Modifications
Benoit Guillemette, Ian Hammond-Martel, Hugo Wurtele, and Alain Verreault
10. Immunofluorescence of Histone Proteins Hilmar Strickfaden and Michael J. Hendzel
11. Acid-Urea Gel Electrophoresis and Western Blotting Of Histones
Catherine A. Hazzalin and Louis C. Mahadevan
12. Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast
Jean Mbogning and Jason C. Tanny
13. A Spiking Strategy for Chip-Chip Data Normalization in S. Cerevisiae
Célia Jeronimo and François Robert
14. High-Resolution Genome-Wide Mapping Of Nucleosome Positioning And Occupancy Level Using Paired-End Sequencing Technology
Mylène Brunelle, Sébastien Rodrigue, Pierre-Étienne Jacques, and Nicolas Gévry
15. Physarum Polycephalum for Studying the Function of Histone Modifications In Vivo
Vanessa Menil-Philippot and Christophe Thiriet
16. A Method for Large Scale Screening of Random Sequence Libraries to Determine the Function of Unstructured Regions from Essential Proteins Jean-François Millau, Benoit Guillemette, and Luc Gaudreau
