Part I: Cell lines with Minimal but Inducible Proviral Expression
1. Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency
Anthony RODARI, Guido Poli, and Carine VAN LINT
2. U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency
Guido Poli
Part II: In vitro and ex vivo primary cell models of minimal but inducible proviral expression
3. An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency
in primary human CD4+ T cells
Rui Li and Fabio Romerio
4. The cultured TCM model of HIV latency
Alberto Bosque
5. A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) MethodMeenakshi Shukla, Fredrick Kizito, Uri Mbonye, Kien Nguyen, Curtis Dobrowolski, and Jonathan Karn
6. TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model
Sydney Bergstresser and Deanna A. Kulpa
7. Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification
Amélie Cattin, Augustine Fert, Delphine Planas, and Petronela Ancuta
8. Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF)
Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, and Carlo Federico Perno
9. Human monocyte-derived macrophages (MDM) - Model 2
Francesca Graziano, Elisa Vicenzi, and Guido Poli
10. Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100
Amelie Bauer
11. Hematopoietic stem and progenitor cells (HSPCs)
Valeri H. Terry, Gretchen E. Zimmerman, Maria C. Virgilio, Mark M. Painter, Dale Bixby, and Kathleen L. Collins
Part III: In vitro and ex vivo tissue-derived models of reversible proviral latency
12. HIV reservoirs: Modeling, Quantification and Approaches to a Cure
Louise A. Ouattara, Nikolas C. Vann, and Gustavo F. Doncel
13. More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication
Jean-Pierre Routy, Franck P. Dupuy, John Lin, and Stéphane Isnard
Part IV: Animal Models
14. Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells
Fernando Real, Yonatan Ganor, and Morgane Bomsel
15. Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond
Nabila Jabrane-Ferrat and Hicham El Costa
16. HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure
Amir Dashti, Vidisha Singh, and Ann Chahroudi
17. Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research
Triana Rivera-Megias, Nhut M Le, and Alonso Heredia
18. Inducing long-term HIV-1 latency in the TKO-BLT mouse model
Yunyun Di and Kerry J. Lavender
Part V: Methods for detection and analysis of the reservoir
19. In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue
Kathleen Busman-Sahay, Michael D. Nekorchuk, Carly Elizabeth Starke, Chi Ngai Chan, and Jacob D. Estes
20. Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH
Mathieu Dubé and Daniel E. Kaufmann
21. Ex vivo differentiat
