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Human Embryonic and Induced Pluripotent Stem Cells

Human Embryonic and Induced Pluripotent Stem Cells


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About the Book

Part I: Maintenance and Cryopreservation of ES and iPS cells

1. Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

Dong-Youn Hwang

2. Growth of Human Embryonic Stem Cells in Long-Term Hypoxia

Vladimir Zachar, Simon C. Weli, Mayuri S. Prasad, and Trine Fink

3. Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein that Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells

Masato Nagaoka and Stephen A. Duncan

4. Scale-Up of Single Cell-Inoculated Suspension Cultures of Human Embryonic Stem Cells

Harmeet Singh, Pamela Mok, and Robert Zweigerdt

5. Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells

Guang-wei Sun, Xiao-xi Xu, Nan Li, Ying Zhang, and Xiao-jun Ma

6. Expansion of Pluripotent Stem Cells in Defined Xeno-Free Culture System

Kristiina Rajala

7. Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells

Behrouz Aflatoonian and Harry D. Moore

8. Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Frida Holm

Part II: Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming

9. Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2

Alessandra Giorgetti, Nuria Montserrat, and Juan Carlos Izpisua Belmonte

10. Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

Naoki Nishishita, Chiemi Takenaka, and Shin Kawamata

11. Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage

Maria V. Shutova, Ilya V. Chestkov, Maria A. Lagarkova, and Sergey L. Kiselev

12. Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells

Hua Liu, Su Mi Choi, and Yoon-Young Jang

13. Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

Kazutoshi Takahashi

14. Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

Hiroe Ohnishi, Yasuaki Oda, and Hajime Ohgushi

15. Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes

Athanasia D. Panopoulos, Sergio Ruiz, and Juan Carlos Izpisua Belmonte

16. Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles

Ann Peters and Elias T. Zambidis

Part III: Generation of Patient-Specific iPS Cells for Clinical Application

17. Generation of iPS Cells from Human Skin Biopsy

Katie Avery and Stuart Avery

18. Generation of Induced Pluripotent Stem Cells from Human Amnion Cells

Masashi Toyoda, Shogo Nagata, Hatsune Makino, Hidenori Akutsu, Takashi Tada, and Akihiro Umezawa

Part IV: Lineage-Specific Differentiation of hES and iPS Cells

19. In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells

Xiaolong Lin, Hua Jiang, Zack Zhengyu Wang, and Tong Chen

20. Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells

K


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Product Details
  • ISBN-13: 9781493958931
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 1st ed. 2012
  • Language: English
  • Returnable: Y
  • Spine Width: 26 mm
  • Weight: 915 gr
  • ISBN-10: 1493958933
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 486
  • Series Title: Springer Protocols Handbooks
  • Sub Title: Lineage-Specific Differentiation Protocols
  • Width: 178 mm


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