Part I: Overview and Health Risk Considerations
1. Immunotoxicology: A Brief History
Kenneth L. Hastings
2. Clinical Immunotoxicology
L. Peyton Myers
3. Investigative Immunotoxicology
Stacey E. Anderson and Hillary L. Shane
4. Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing
Annalise N. vonderEmbse and Jamie C. DeWitt
5. Markers of Inflammation
Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, and Ellen Evans
Part II: Legacy Protocols: In Vivo
6. The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)
Gregory S. Ladics
7. Methylated-Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice
Jessica Margaret Sido
8. Use of the LLNA: BrdU-ELISA for Skin Sensitization Hazard Assessment
David M. Lehmann
9. Host Resistance Assays
Stefanie C.M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, and Robert W. Luebke
10. Enhanced Histopathology Evaluation of Lymphoid Organs
Susan A. Elmore
11. Tumor Challenges in Immunotoxicity Testing
Sheung Ng and Judith T. Zelikoff
Part III: Legacy Protocols: Ex Vivo and In Vitro
12. Flow Cytometry for the Immunotoxicologist
Scott T. Espenchied, Robert M. Tighe, and Kymberly M. Gowdy
13. Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T Lymphocyte Assay
Gary R. Burleson, Florence G. Burleson, and Rodney R. Dietert
14. Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing
Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, and Prakash S. Nagarkatti
15. Natural Killer (NK) Cell Assays in Immunotoxicity Testing
Qing Li
16. Dendritic Cell Assays
Joanna M. Kreitinger and David M. Shepherd
17. Evaluating Macrophages in Immunotoxicity Testing
Jennifer Franko, Jamie L. McCall, and John B. Barnett
18. Evaluating Cytokines in Immunotoxicity Testing
Emanuela Corsini and Robert V. House
Part IV: Alternative Protocols
19. Functional Assays of Hematopoietic Stem Cells in Tox