Standard Influenza protocols and surveillance
1. Influenza Virus isolation, preparation and titration
2. Vaccine selection and preparation
3. Isolation of clinical strains, sequencing, and reverse genetics
4. Diagnosis
5. Temporal-spatial analysis and real time surveillance of virus outbreaks (i.e. Ebola)
6. How the general population, internet, SNS helps predict epidemics (Overview)
Systems approach to discover Influenza virus-host interactions
7. Integrated Omics and Systems Virology (Overview)
8. Computational Biology of Infectious Diseases and study of virus evolution
9. Haploid Screening for host factors in virus infection
10. Proteomics, and affinity-MS virus interactome
11. Influenza Virus Replicon system in Yeast
12. Virus Receptor Hunting using Ligand Based Receptor Capture
13. Fully-automated screen using FDA-approved drug libraries
Dissecting cell biology of Influenza infection
14. Cell Biology of influenza virus-host interactions (Overview)
15. Influenza virus entry assays in tissue culture cells
16. DC-SIGN mediated virus uptake analysis
17. In vitro assay to detect virus disassembly during endocytic transit
18. Endosome fractionation and detection of viral and cellular antigens
19. Virus-liposome fusion assays using cryo EM
20. Visualizing influenza capsid uncoating in the cytoplasm of cells using combined cryo EM, SEM, and aqueous AFM
21. Quantitative PCR based analysis of Influenza virus endocytic escape
22. Virus uncoating detection using beta lactamase fused VLPs
23. Purification of viral polymerase using MultiBac system for structural biology
24. Influenza polymerase transcription analysis - vRNA vs cRNA
25. Image Analysis: From Single Cell analysis to High throughput imaging (Overview)
26. Single molecule FISH of incoming and egressing influenza vRNPs
27. Live imaging of replication and egress using a GFP influenza virus
28. Virus fluorescent labeling for imaging and super-resolution microscopy
29. Cryo EM of influenza virus progeny during budding
30. Assessing virus growth in cells depleted of host factors with siRNA or CRISPR/Cas9
Influenza animal models, physiological systems, and immunology
31. Animal models: mice, ferrets, pigs, monkeys and CRISPR animals (Overview)
32. IAV in vivo imaging, virus titration and immune response analysis
33. MxA and interferon (Overview)
34. Clinical bacteria and viral co-infection studies
35. 3D ex vivo culture system to study virus infection and entry
