1. The Application of Humanized Mouse Models for the Study of Human Exclusive Viruses
Fatemeh Vahedi, Elizabeth C. Giles, and Ali A. Ashkar
2. Zebrafish as a Model for the Study of Host-Virus Interactions
Peng Fei Zou and Pin Nie
3. Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Non-Radioactive Oligo Probes
Tianyun Long and Rui Lu
4. Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin
Chandra S. Mukhopadhyay, Ramneek Verma, and Jasdeep Singh
5. Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods
Sarah J. Poynter and Stephanie J. DeWitte-Orr
6. RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity
Renee C. Ireton, Courtney Wilkins, and Michael Gale, Jr.
7. Methods to Visualize MAVS Subcellular Localization
Christine Vazquez, Dia C. Beachboard, and Stacy M. Horner
8. Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture
Alice Mayer, Jonathan Maelfait, Anne Bridgeman, and Jan Rehwinkel
9. cGAMP Quantification in Virus-Infected Human Monocyte-Derived Cells by HPLC-Coupled Tandem Mass Spectrometry
Jennifer Paijo, Volkhard Kaever, and Ulrich Kalinke
10. Methods of Assessing STING Activation and Trafficking
Vladislav Pokatayev and Nan Yan
11. Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells
Shiyou Zhu, Yuexin Zhou, and Wensheng Wei
12. High Throughput Screening for Identification of Novel Innate Immune Activators
Bryan J. Gall and Victor R. DeFilippis
13. Chromosome Conformation Capture for Research on Innate Antiviral Immunity
Yoon Jung Kim and Tae Hoon Kim
14. Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing
Gabriel A. Leiva-Torres, Nestor Nebesio, and Silvia M. Vidal
15. Isolation, Purification, and Culture of Primary Murine Sensory Neurons
Sarah Katzenell, Jorge R. Cabrera, Brian J. North, and David A. Leib
16. Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs
Claudia U. Duerr and Jörg H. Fritz
17. Epidemiological Methods
Biao Wang and Mark Loeb