1. General Introduction: Recombinant protein production and purification of insoluble proteins
Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, and Elena García-Fruitós
Part I: Prokaryotic Expression Systems
2. Overcoming the solubility problem in E. coli: available approaches for recombinant protein production
Claudia Ortega, Pablo Oppezzo, and Agustín Correa
3. A3D 2.0 update for the prediction and optimization of protein solubility
Jordi Pujols, Valentín Iglesias, Jaime Santos, Aleksander Kuriata, Sebastian Kmiecik, and Salvador Ventura
4. Tailoring codon usage to the underlying biology for protein expression optimization
Zahra Alirezaeizanjani, Jan-Hendrik Trösemeier, Christel Kamp, and Sophia Rudorf
5. Protein Expression Optimization Strategies in E. coli: A Tailored Approach in Strain Selection and Parallelizing Expression Conditions
Shyn Ric Tang, Balaji Somasundaram, and Linda H.L. Lua
6. A novel approach for production of aggregation prone proteins using the spidroin-derived NT* tag
Nina Kronqvist, Anna Rising, and Jan Johansson
7. Cleavable Self-Aggregating Tags (cSAT) for Therapeutic Peptide Expression and Purification
Xiaofeng Yang, Zhanglin Lin, and Yanyun Jing
8. Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility
Shuxiong Chen and Bernd Rehm
9. Introduction of a hexalysine (6K) tag can protect from N-terminal cleavage and increase yield of recombinant proteins expressed in the periplasm of E. coli
Vasiliki Paraskevopoulou, Mohammed Alissa, Naim Hage, and Franco H. Falcone
10. Two-tiered selection and screening strategy to increase functional enzyme production in Escherichia coli
Jason T. Boock, May Taw, Brian C. King, Robert J. Conrado, Donna M. Gibson, and Matthew P. DeLisa
11. Method for production of cysteine-rich proteins in Lactococcus lactis expression system Susheel K. Singh and Vandana Singh
12. Heterologous protein production in Lactobacillus (plantarum) using pSIP vectors
Geir Mathiesen, Lars Axelsson, Vincent GH Eijsink
13. Soluble recombinant protein production in Pseudoaltermonas haloplanktis TAC125: the case study of the full-length human CDKL5 protein
Marzia Calvanese, Andrea Colarusso, Concetta Lauro, Ermenegilda Parrilli and Maria Luisa Tutino
14. A protocol to enhance soluble protein expression in the cytoplasm of Bacillus subtilis
Hoang D Nguyen and Trang TP Phan
15. Applications of cell-free synthesized membrane protein precipitates
Julija Mezhyrova, Karsten Mörs, Clemens Glaubitz, Volker Dötsch, and Frank Bernhard
Part II: Eukaryotic Expression Systems
16. High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of "difficult-to-express" secretory recombinant proteins
Yasuaki Kawarasaki, Takeshi Kurose, Sayaka Ohashi, Runa Watabe, Mizuki Tanaka, and Keisuke Ito
17. Insect Cells-Baculovirus system for the production of difficult to express proteins: from expression screening for soluble constructs to protein quality control
Simon Pichard, Nathalie Troffer-Charlier, Isabelle Kolb-Cheynel, Pierre Poussin-Courmontagne, Wassim Abdulrahman, Catherine Birck, Vincent Cura, and Arnaud Poterszman
18. Transient gene expression in human Expi293 cells
Haroldo Cid da Silva Junior
