Part I: Analysis of mRNA Translation Regulation

1. An Overview of Methods for Detecting eIF2α Phosphorylation and the Integrated Stress Response

Agnieszka Krzyzosiak, Aleksandra Pitera, and Anne Bertolotti

2. CRISPR-Based Screening for Stress Response Factors in Mammalian Cells

Xiaoyan Guo and Martin Kampmann

3. Multiplexed Analysis of Human uORF Regulatory Functions during the ISR Using PoLib-Seq

Gemma E. May and C. Joel McManus

4. Measuring Bulk Translation Activity in Single Mammalian Cells during the Integrated Stress Response

Alyssa M. English and Stephanie L. Moon

5. Quantitative Translation Proteomics Using mePROD

Kevin Klann and Christian Münch

6. Quantifying the Binding of Fluorescently Labelled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2

Martin D. Jennings and Graham D. Pavitt

7. Mammalian In Vitro Translation Systems

Yulia Gonskikh, Valentina Pecoraro, and Norbert Polacek

8. Measuring Repeat-Associated Non-AUG (RAN) Translation

Shaopeng Wang and Shuying Sun

9. Analysis of Ribosome Profiling Data

Carine Legrand, Khanh Dao Duc, and Francesca Tuorto

10. Analysis of Translational Control in the Integrated Stress Response by Polysome Profiling

Michael J. Holmes, Jagannath Misra, and Ronald C. Wek

11. High-Resolution Ribosome Profiling for Determining Ribosome Functional States during Translation Elongation

Mohammad Shafieinouri, Britnie Santiago Membreno, and Colin Chih-Chien Wu

12. Fluorescence Intensity-Based eIF2B's Guanine Nucleotide-Exchange Factor Activity Assay

Yusuke Sekine, David Ron, and Alisa F. Zyryanova

Part II: Analysis of Interaction Networks and RNP Granules

13. Collective Learnings of Studies of Stress Granule Assembly and Composition

Hadjara Sidibé and Christine Vande Velde

14. Detecting Stress Granules in Drosophila Neurons

Fabienne De Graeve, Nadia Formicola, Kavya Vinayan Pushpalatha, Akira Nakamura, Eric Debreuve, Xavier Descombes, and Florence Besse

15. Monitoring and Quantification of the Dynamics of Stress Granule Components in Living Cells by Fluorescence Decay after Photoactivation

Anna-Carina Söhnel, Nataliya I. Trushina, and Roland Brandt

16. Probing Protein Solubility Patterns with Proteomics for Insight into Network Dynamics

Xiaojing Sui,