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Integrin and Cell Adhesion Molecules

Integrin and Cell Adhesion Molecules


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International Edition


About the Book

Part I: Basic Protocols for the Study of Integrin and Related Cell Adhesion Molecule Functionality In Vitro

1. Overview: Assays for Studying Integrin-Dependent Cell Adhesion

Alexandre Chigaev and Larry A. Sklar

2. Cell Adhesion Assays

Gabriele Weitz-Schmidt and Stéphanie Chreng

3. Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes

Ziv Shulman and Ronen Alon

4. Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes

Daliya Banerjee and Motomu Shimaoka

5. Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands

Thomas Vorup-Jensen

6. Cell-Free Ligand-Binding Assays for Integrin LFA-1

Koichi Yuki

Part II: Structural Biology Approaches for Studying Integrins and Related Cell Adhesion Molecules

7. Overview: Structural Biology of Integrins

Guanyuan Fu, Wei Wang, and Bing-Hao Luo

8. Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography

Hongmin Zhang and Jia-huai Wang

9. Electron Microscopic Imaging of Integrin

Kenji Iwasaki

10. An NMR Method to Study Protein-Protein Interactions

Noritaka Nishida and Ichio Shimada

11. Single Molecule Methods to Study Cell Adhesion Molecules

Joonil Seog

Part III: Imaging for Investigating Cell Adhesion and Migration

12. Overview: Imaging in the Study of Integrins

Christopher V. Carman

13. Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals

Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher

14. Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer

Craig T. Lefort, Young-min Hyun, and Minsoo Kim

15. High-resolution Fluorescence Microscopy to Study Transendothelial Migration

Christopher V. Carman

16. Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes

Thomas T. Murooka and Thorsten R. Mempel

Part IV: Signaling through Cell Adhesion Molecules

17. Overview of Integrin Signaling in the Immune System

Tatsuo Kinashi

18. Rap1 and Integrin Inside-out Signaling

Koko Katagiri and Tatsuo Kinashi

19. Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis

Jean-Cheng Kuo, Xuemei Han, John R. Yates III, and Clare M. Waterman

20. Talin and Signaling Through Integrins

Mohamed Bouaouina, David S. Harburger, and David A. Calderwood

21. Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins

Yingchun Wang and Richard L. Klemke

Part V: Cell Adhesion and Migration at Organismal Levels in a Physiological Context

22. Overview: Studying Integrins In Vivo

Clifford A. Lowell and Tanya N. Mayadas

23. A Method for the Generation of Conditional Gene-Targeted Mice

Masahiro Yamamoto and Kiyoshi Takeda

24. T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations

Jaime De Calisto, Eduardo J. Villablanca, Sen Wang, Maria R. Bono, Mario Rosemblatt, and J. Rodrigo Mora

25. In Vivo Quantitative Proteomics: the SILAC-Mouse

Sara Zanivan, Marcus Krueger, and Matthias Mann

26. Analysis of Chemotaxis in Dictyostelium

Huaqing Ca

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Product Details
  • ISBN-13: 9781493958085
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 1st ed. 2012
  • Language: English
  • Returnable: Y
  • Spine Width: 28 mm
  • Weight: 988 gr
  • ISBN-10: 1493958089
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 529
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm

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