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Ion Channel Reconstitution

Ion Channel Reconstitution


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About the Book

It is now over 30 years since the idea of ion-conducting pores burst on the elec- trophysiological scene, 15 years since these were generalIy realized to be mem- brane-spanning proteins, and 10 years since the first observations of single ion channels from higher organisms were made. During the past 5 years, several integral membrane channel proteins have been purified in a functionalIy competent state: the nicotinic acetylcholine receptor, the Na + channel, mitochondrial "VDAC," and a variety of porins. The stage is thus set to attack ion channels in the same ways that biochemists have been attacking enzymes for decades: isolation folIowed by functional analysis in as simple a system as possible, with a view towards understanding the molecular mechanisms ofthe protein's behavior and how this is related to the underlying molecular structure. This is always a daunting task, alI the more so with ion channels because of our still primitive and scanty understanding of channel structures and because of the difficulty in iso- lating functionally active channel proteins. In this volume, which can be considered a biochemically slanted companion to Sakmann and Neher's Single-Channel Recording, I have tried to present a view of the current landscape of ion-channel reconstitution. These chapters illustrate not only the different approaches and techniques of the major practitioners of ion- channel reconstitution but, as importantly, the varied motivations for doing this kind of work.


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Product Details
  • ISBN-13: 9781475713633
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Depth: 38
  • Height: 244 mm
  • No of Pages: 577
  • Series Title: English
  • Weight: 992 gr
  • ISBN-10: 1475713630
  • Publisher Date: 28 Jan 2013
  • Binding: Paperback
  • Edition: 1986 ed.
  • Language: English
  • Returnable: Y
  • Spine Width: 31 mm
  • Width: 170 mm


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