Part I: Physiology
1. Phenotyping Jasmonate Regulation of Senescence
Martin A. Seltmann and Susanne Berger
2. Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis
Bryan Thines, Ajin Mandaokar, and John Browse
3. Phenotyping Jasmonate Regulation of Root Growth
Fabian Kellermeier and Anna Amtmann
Part II: Environment
4. Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria
Saskia C.M. Van Wees, Johan A. Van Pelt, Peter A.H.M. Bakker, and Corné M.J. Pieterse
5. Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory
Marco Herde, Abraham J.K. Koo, and Gregg A. Howe
6. Pseudomonas syringae Infection Assays in Arabidopsis
Jian Yao, John Withers, and Sheng Yang He
7. Jasmonate Signaling in the Field, Part I: Elicited Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations
Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin
8. Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural Nicotiana attenuata Populations
Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin
Part III: Molecules
9. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves
Yusuke Jikumaru, Mitsunori Seo, Hideyuki Matsuura, and Yuji Kamiya
10. A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding
Gaëtan Glauser and Jean-Luc Wolfender
11. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach
Bettina Hause, Kati Mielke, and Susanne Forner
12. Jasmonic Acid-Amino Acid Conjugation Enzyme Assays
Martha L. Rowe and Paul E. Staswick
13. Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins
Sandra Fonseca and Roberto Solano
14. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins
Amparo Cuéllar Pérez, Laurens Pauwels, Rebecca de Clercq, and Alain Goossens
15. Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins
Tiancong Qi, Susheng Song, and Daoxin Xie
16. Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling
Volkan Çevik and Kemal Kazan
17. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA
Johan Memelink
18. Transient Expression Assays in Tobacco Protoplasts
Robin Vanden Bossche, Brecht Demedts, Rudy Vanderhaeghen, and Alain Goossens
19. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters
Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki
20. Expression Analysis of Jasmonate-Responsive Lectins in Plants
Nausicaä Lannoo and Els J.M. Van Damme
Part IV: Omics
21. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics
Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte
22. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
