PART I: STANDARD TECHNIQUES
1. History of Plant Lectin Research
Els J.M. Van Damme
2. Fungal Lectins, A Growing Family
Yuka Kobayashi, and Hirokazu Kawagishi
3. The "White Kidney Bean Incident" in Japan
Haruko Ogawa, and Kimie Date
4. Hemagglutination (Inhibition) Assay
Kotone Sano, and Haruko Ogawa
5. Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources
Kimie Date and Haruko Ogawa
6. High-Performance Lectin Affinity Chromatography
Yuka Kobayashi
7. Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography
Katsuko Yamashita, and Takashi Ohkura
8. Lectin-Probed Western Blot Analysis
Takeshi Sato
9. Solid-Phase Assay of Lectin Activity Using HRP-conjugated Glycoproteins
Kyoko Kojima-Aikawa
10. A Simple Viral Neuraminidase-based Detection for High-throughput Screening of Viral Hemagglutinin-host Receptor Specificity
Nongluk Sriwilaijaroen, and Yasuo Suzuki
11. Lectin Affinity Electrophoresis
Yuka Kobayashi
12. Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis between Lectins and Glycans
Mitsuhiro Kinoshita, and Kazuaki Kakehi
13. Basic Procedures for Lectin Flow Cytometry
Kenta Moriwaki, and Eiji Miyoshi
14. Histochemical Staining Using Lectin Probes
Yoshihiro Akimoto, and Hayato Kawakami
15. Equilibrium Dialysis using Chromophoric Sugar Derivatives
Tomomitsu Hatakeyama
16. Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis between Lectins and Sugars
Kanji Hori and Makoto Hirayama
17. Surface Plasmon Resonance as a Tool to Characterize Lectin-carbohydrate Interactions
Yasuro Shinohara, and Jun-ichi Furukawa
18. Isothermal Calorimetric Analysis of Lectin-sugar Interaction Yoichi Takeda and Ichiro Matsuo
19. Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines
Mamoru Mizuno
PART II: EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS
20. Lectin-Based Glycomics: How and When Was the Technology Born?
Jun Hirabayashi
21. Frontal Affinity Chromatography (FAC): Theory and Basic Aspects
Ken-ichi Kasai
22. Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides Chihiro Sato
23. Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation. From Sample Pretreatment to Data Standardization
Atsushi Kuno, Atsushi Matsuda, Sachiko Unno, Binbin Tan, Jun Hirabayashi, and Hisashi Narimatsu
24. Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces
Azusa Shibazaki, and Tohru Gonoi
25. Application of Lectin Microarray to Bacteria Including Lactobacillus casei /paracasei Strains
Emi Yasuda, Tomoyuki Sako, Hiroaki Tateno, and Jun Hirabayashi
26. Live Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN
Hiroaki Tateno, Yasuko Onuma, and Yuzuru Ito
27. Carbohydrate-binding Specificity of Lectins Using Multiplexed Glyco-bead Array
Kazuo Yamamoto
28. Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins
Yu-ki Matsuno, and Akihiko Kameyama
PAPRT III: TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS
29. Over
