Part 1. Identification and Typing of Legionella in the Environment and During Disease

1. Identification of Legionella in the Environment

Natalia A. Kozak, Claressa E. Lucas, and Jonas M. Winchell

2. Identification of Legionella in Clinical Samples

Sophe Jarraud, Ghislaine Descours, Christophe Ginevra, Gerard Lina, and Jerome Etienne

3. Sequence-Based Identification of Legionella

Rodney M. Ratcliff

4. Investigation of Outbreaks - Epidemiology

Carol Joseph

5. Outbreak Investigations and Identification of Legionella in Contaminated Water

Susanne Lee and John Lee

6. Typing Methods for Legionella

Christian Lück, Norman K. Fry, Jürgen H. Helbig, Sophie Jarraud, and Timothy G. Harrison

Part 2. Physiology and genetics of Legionella

7. Culture, Media and Handling of Legionella

Christa H. Chatfield and Nicholas P. Cianciotto

8. The Intracellular Metabolism of Legionella by Isotopologue Profiling

Klaus Heuner and Wolfgang Eisenreich

9. Induction of Competence for Natural Transformation in Legionella pneumophila and Exploitation for Mutant Construction

Carmen Buchrieser and Xavier Charpentier

10. Constructing Unmarked Gene Deletions in Legionella pneumophila

Andrew Bryan, Zachary D. Abbot, and Michele Swanson

11. Natural Biofilm Formation with Legionella pneumophila

Emilie Portier and Yann Héchard

12. Production of Legionella pneumophila Monospecies Biofilms in a Low-Nutrient Concentration Medium

Sophie Pécastaings and Christine Roques

13. Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila

Jens Jäger and Michael Steinert

Part 3. Cellular microbiology of Legionella

14. Analysis of Legionella Infection by Flow Cytometry

Andre Tiaden, Aline Kessler, and Hubert Hilbi

15. Analysis of Legionella Infection Using RNAi in Drosophila Cells

Dennise A. De Jesús, Tamara J. O'Connor, and Ralph R. Isberg

16. Immunofluorescence Imaging of Legionella Infection In vitro

Stefan S. Weber and Annette Oxenius

17. Electron Microscopy of Legionella and Legionella-Infected Cells

Gary Faulkner and Raphael Garduno

18. Pathogen Vacuole Purification from Legionella-Infected Amoeba and Macrophages

Christine Hoffmann, Ivo Finsel, and Hubert Hilbi

19. Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System

Wenhan Zhu and Zhao-Qing Luo

20. Subcellular Localization of Legionella Dot/Icm Effectors

Adam J. Vogrin, Aurelie Mousnier, Gad Frankel, and Elizabeth L. Hartland

Part 4. Biochemical assays to study Legionella effectors and enzymes

21. Purification and Characterization of Legionella U-Box-Type E3 ubiquitin ligase

Hiroki Nagai and Tomoko Kubori

22. Legionella Phospholipases Implicated in Infection - Determination of Enzymatic Activities

Kathleen Seipel and Antje Flieger

23. Interactions of Legionella Effector Proteins with Host Phosphoinositide Lipids

Stephen Weber, Stephanie Dolinsky, and Hubert Hilbi

24. Characterization of Legionella lipopolysaccharide

Christian Lück and Jürgen Helbig

Part 5. Immunity and host response against Legionella

25. The Tetrahymena and Acanthamoeba Model Systems

Sharon G. Berk and Rafael A. Garduño

26. Dictyostelium Host Response to Legionella infection: Strategies and Assays

Salvatore Bozzaro, Barbara Peracino, and Ludwig Eichinger

27. The Caenorhabditis elegans Model of Legionella Infection

Ann Karen C. Brassinga and Costi D. Sifri