Part 1 - Structural and Biochemical Analysis of LINC complexes

1. EM Tomography of LINC Complexes

Marie-Christin Spindler, Frederick Helmprobst, Christian Stigloher and Ricardo Benavente

2. Recombinant Purification of the Periplasmic Portion of the LINC Complex

Victor E. Cruz and Thomas U. Schwartz

3. Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

Chloe Potter and Didier Hodzic

4. Analysis of Nesprin-2 Interaction with its Binding Partners and Actin

Susumu Antoku and Gregg G. Gundersen

5. Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID

Brian Burke

Part 2 - Mechanical Aspects of the LINC Complex

6. Using Nesprin Tension Sensors to Measure Force on the LINC Complex

Paul T. Arsenovic and Daniel E. Conway

7. Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei

Nejma Belaadi, Angelique Millon-Frémillon, Julien Aureille, and Christophe Guilluy

8. Direct Force Probe for Nuclear Mechanics

9. Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning

Ruijun Zhu and Gregg G. Gundersen

10. Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments

Jeremy Keys, Aaron Windsor, and Jan Lammerding

Part 3 - Analysis of the LINC Complex in Model Systems and Development

11. Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy

Jared Hennen, Isaac Angert, Kwang-Ho Hur, G.W. Gant Luxton, and Joachim D. Mueller

12. Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging

Jay R. Unruh, Brian D. Slaughter, and Sue L. Jaspersen

13. Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans

Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, and Daniel A. Starr

14. High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development

15. Computational Methods for Studying the Plant Nucleus

Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann, and David E. Evans

Maxime Voisin, Emmanuel Vanrobays, and Christophe Tatout

17. Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components

Javier Varas and Mónica Pradillo