Part I: Overview of Lipases, Phospholipases and Related Enzymes

1. Lipases: An Overview Leticia Casas Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval and Alain Marty

2. Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification Yassine Ben Ali, Robert Verger, and Abdelkarim Abousalham

3. Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus Mónica Sánchez-González, Allan Blanco-Gámez, Roberto Parra-Saldívar, Juan Carlos Mateos-Díaz and Maria Isabel Estrada Alvarado

4. Phospholipases: An Overview Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri and Michael H. Gelb

Part II: Screening and Sources of Lipases, Phospholipases and Its Inhibitors

5. High-Throughput Sceening Method for Lipases/Esterases Eduardo Mateos-Díaz, Jorge Alberto Rodríguez, María de los Ángeles Camacho-Ruiz, Juan Carlos Mateos-Díaz

6. Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries

Dolores Reyes-Duarte, Manuel Ferrer, and Humberto García-Arellano

7. Plant Lipases: Partial Purification of C. papaya Lipase

Ivanna Rivera, Juan Carlos Mateos-Díaz, and Georgina Sandoval

8. Plant Phospholipases: an Overview

Geliang Wang, Stephen Ryu, and Xuemin Wang

9. Lipase and Phospholipase Inhibitors: Design and Applications

Mark E. Venable

10. High-Throughput Assay of Secreted Phospholipases A2 Inhibitors

Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing and Michael H. Gelb

Part III Cloning, Production, Purification and Characterization of Lipases and Phospholipases

11. Heterologous Expression Systems for Lipases: A Review

Francisco Valero

12. Streptomyces Phospholipase D: Cloning and Production

Yozo Nakazawa

13. Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses

Julian Gomez-Cambronero and Karen M. Henkels

14. Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite

Susana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres

15. Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27

Pablo Fuciños, Ramón González, Estrella Atanes, Ana Belem Fernández Sestelo, Nelson Pérez-Guerra, Lorenzo Pastrana and María Luisa Rúa

16. Purification, Biochemical and Kinetic Properties of Recombinant
Staphylococcus aureus Lipase

Habib Horchani, Ahmed Fendri, Hanen Louatia, Adel Sayari, Youssef Gargouri and Robert Verger

17. Phospholipase A2 Purification and Characterization; A Case Study

Aida Karray, Youssef Gargouri, Robert Verger and Sofiane Bezzine

Part IV: Optimization of the Biocatalyst and Reaction Conditions

18. The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Lipases

Sophie Duquesne, Florence Bordes, Franck Fudalej, Jean-Marc Nicaud and Alain Martya

19. Molecular Modeling of Lipase Binding to a Substrate-Water Interface

Christian C. Gruber and Jürgen Pleiss

20. Different Strategies for Hyperactivation of Lipase Biocatalysts

Jose M. Palomo and Jose M. Guisan

21. Rational Design of Immobilized Lipases and Phospholipases

Alberto del Monte Martínez and Bessy V. Cutiño Avila

22. Thermodynamical Methods for the Optimization of Lipase Catalyzed Reactions

Edmundo Castillo, Alejandro Torres-Gavilán, Georgina Sandoval, Alain Marty

Part V: Applications of Lipases and Phopholipases

23. Lipases as Biocatalysts for the