Part I: RNA and Gene Expression
1. Cloning Full-Length Transcripts and Transcript Variants Using 5' and 3' RACE
Lita A. Freeman
2. Monitoring Gene Expression: Quantitative Real-Time RT-PCR
Elke M. Wagner
3. Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases
Nalini Raghavachari
4. Northern Analysis of Gene Expression
Lita A. Freeman
5. Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
Jonathan E. Feig and Edward A. Fisher
Part II: Sequencing
6. Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research
Victoria Youngblood and James G. Taylor VI
7. Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research
Lena Diaw, Victoria Youngblood, and James G. Taylor VI
Part III: Transgenic, Knockout, and Knockdown Methodologies
8. Strategies for Designing Transgenic DNA Constructs
Chengyu Liu
9. Purification of Plasmid and BAC Transgenic DNA Constructs
Chengyu Liu, Yubin Du, Wen Xie, and Changyun Gui
10. Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production
Chengyu Liu, Wen Xie, Changyun Gui, and Yubin Du
11. Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes
Boris Vaisman
12. Generation of General and Tissue-Specific Gene Knockout Mouse Models
Xian-Cheng Jiang
13. Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism
William R. Lagor, Julie C. Johnston, Martin Lock, Luk H. Vandenberghe, and Daniel J. Rader
14. Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology
Rosanne M. Crooke and Mark J. Graham
Part IV: Special Topics
15. Chromatin Immunoprecipitation
Grant D. Barish and Rajenda K. Tangirala
16. Measurement of Lecithin: Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate
Boris Vaisman and Alan T. Remaley
17. Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis
Lita A. Freeman
18. Western Blots
Lita A. Freeman
