Part I. Parasite Culturing

1. In vitro Culturing P. falciparum Erythrocytic Stages

Alexander G. Maier and Melanie Rug

2. Production of Plasmodium falciparum Gametocytes in vitro

Kathryn Shaw Saliba and Marcelo Jacobs-Lorena

3. In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes

Anil K. Ghosh and Marcelo Jacobs-Lorena

4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages

Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken

5. Laboratory Maintenance of Rodent Malaria Parasites

Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden

Part II. Genome Manipulation

6. Transfection of Plasmodium falciparum

Melanie Rug and Alexander G. Maier

7. Transfection of Rodent Malaria Parasites

Nisha Philip, Rachael Orr, and Andrew P. Waters

8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNA

Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker

9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database

Shahid M. Khan, Hans Kroeze, Blandine Franke‐Fayard, and Chris J. Janse

10. Transient Transfection of Plasmodium vivax Blood-stage Parasites

Cecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer

11. Silencing of Genes and Alleles by RNAi in Anopheles gambiae

Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin

Part III. 'OMIC' Techniques

12. Analysis of Variant Gene Family Expression by Quantitative PCR

Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira

13. DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites

Zbynek Bozdech, Sachel Mok, and Archna P. Gupta

14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform

Heather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás

15. Transcriptome Analysis using RNA-seq

Wieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg

16. Experimental Tools for the Study of Protein Phosphorylation in Plasmodium

Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig

17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic Analysis

Kellen L. Olszewski and Manuel Llinás

Part IV. Red Blood Cell Stages

18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum

David T. Riglar and Jake Baum

19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites

Shailja Singh and Chetan E. Chitnis

20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability

Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet

21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events

Ang Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell

22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System

Anand Srivastava, Yves Durocher, and Benoît Gamain

23. Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium

Jose-Juan Lopez-Rubio