1. Studying Recombination in Mouse Oocytes
Xianfei Sun and Paula E. Cohen
2. Analysis of Recombinants in Female Mouse Meiosis
Esther de Boer, Maria Jasin, and Scott Keeney
3. Studying Meiosis Specific Cohesins in Mouse Embryonic Oocytes
Ji-hye Kim, Kei-ichiro Ishiguro, Nobuaki Kudo, and Yoshinori Watanabe
4. Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development
Jing He, Andrew J. Childs, Jieqian Zhou, and Richard A. Anderson
5. Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos
Jacek Z. Kubiak
6. Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes
Janet E. Holt, Simon I.R. Lane, and Keith T. Jones
7. Using FRET to Study RanGTP Gradients in Live Mouse Oocytes
Julien Dumont and Marie-Hélène Verlhac
8. Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in Oocytes
Mark Levasseur
9. RNAi-Based Methods for Gene Silencing in Mouse Oocytes
Paula Stein, Petr Svoboda, and Richard M. Schultz
10. Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes
Petros Marangos
11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
Jay M. Baltz, Hannah E. Corbett, and Samantha Richard
12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes
Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer
13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang
14. Chromosome Spreads with Centromere Staining in Mouse Oocytes
Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann
15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Lynne Anguish and Scott Coonrod
16. Measuring Ca2+ Oscillations in Mammalian Eggs
Karl Swann
17. Counting Chromosomes in Intact Eggs
Teresa Chiang and Michael A. Lampson
18. Free-Hand Bisection of Mouse Oocytes and Embryos
Zbigniew Polanski and Jacek Z. Kubiak
19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
Souraya Jaroudi and Dagan Wells
20. Nuclear Transfer in the Mouse Oocyte
Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama
Petros Marangos
11. Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos
Jay M. Baltz, Hannah E. Corbett, and Samantha Richard
12. Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes
Solon Riris, Suzanne Cawood, Paul Serhal, and Hayden Homer
13. Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
Kuo-Tai Yang, Yi-Nan Lin, Shu-Kuei Li, and Tang K. Tang
14. Chromosome Spreads with Centromere Staining in Mouse Oocytes
Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann
15. Preparation of Mammalian Oocytes for Transmission Electron Microscopy
Lynne Anguish and Scott Coonrod
16. Measuring Ca2+ Oscillations in Mammalian Eggs
Karl Swann
17. Counting Chromosomes in Intact Eggs
Teresa Chiang and Michael A. Lampson
18. Free-Hand Bisection of Mouse Oocytes and Embryos
Zbigniew Polanski and Jacek Z. Kubiak
19. Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
Souraya Jaroudi and Dagan Wells
20. Nuclear Transfer in the Mouse Oocyte
Eiji Mizutani, Ats
