PART I Expression and Purification of Matrix Metalloproteases
1. Expression and Purification of Matrix Metalloproteinases in Escherichia coli
Krishna K. Singh, Ruchi Jain, Harini Ramanan, and Deepak K. Saini
2. Expression and Purification of a Matrix Metalloprotease Transmembrane Domain in Escherichia coli
Charles A. Galea
3. Heterologous Expression of the Astacin Protease Meprin b in Pichia pastoris
Dagmar Schlenzig and Stephan Schilling
PART II Structural Characterization of Matrix Metalloproteases
4. Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction
Elena Decaneto, Wolfgang Lubitz, and Hideaki Ogata
5. Mapping Lipid Bilayer Recognition Sites of Metalloproteinases and other Prospective Peripheral Membrane Proteins
Tara C. Marcink, Rama K. Koppisetti, Yan G. Fulcher, and Steven R. Van Doren
6. Using Small Angle X-ray Scattering (SAXS) to Characterise the Solution Conformation and Flexibility of Matrix Metalloproteinases (MMPs)
Louise E. Butt, Robert A. Holland, Nikul S. Khunti, Debra L. Quinn, and Andrew R. Pickford
PART III Computational Simulations of Matrix Metalloproteases
7. Molecular Dynamics Studies of Matrix Metalloproteases
Natalia Díaz and Dimas Suárez
PART IV Determining Matrix Metalloprotease Substrate Specificity
8. Determining the Substrate Specificity of Matrix Metalloproteases using Fluorogenic Peptide Substrates
Maciej J. Stawikowski, Anna M. Knapinska, and Gregg B. Fields
9. Time-resolved Analysis of Matrix Metalloproteinase Substrates in Complex Samples
Pascal Schlage, Fabian E. Egli, and Ulrich auf dem Keller 10. Identification of Protease Cleavage Sites by Charge-Based Enrichment of Protein N-termini
Zon W. Lai and Oliver Schilling
11. Mapping the Substrate Recognition Landscapes of Metalloproteases using Comprehensive Mutagenesis
Colin A. Kretz
PART V Detection of Matrix Metalloproteases
12. Detection of Matrix Metalloproteinases by Zymography
Rajeev B. Tajhya, Rutvik S. Patel, and Christine Beeton
13. Imaging Matrix Metalloproteases in Spontaneous Colon Tumors: Validation by Correlation with Histopathology
Harvey Hensley, Harry S. Cooper, Wen-Chi L. Chang, and Margie L. Clapper
PART VI Matrix Metalloprotease Inhibitors 14. Virtual High-throughput Screening for Matrix Metalloproteinase Inhibitors Jun Yong Choi, and Rita Fuerst 15. Computational Approaches to Matrix Metalloprotease Drug Design Tanya Singh, B. Jayaram, and Olayiwola Adedotun Adekoya
16. A Simple, Adaptable Blood-Brain Barrier Cell Model for Screening Matrix Metalloproteinase Inhibitor Functionality
Jennifer S. Myers, Joan Hare, and Qing-Xiang Amy Sang
PART VII Matrix Metalloproteases as Biomarkers
17. Matrix Metalloproteases as Biomarkers of Disease
Fernando Luiz Affonso Fonseca, Beatriz da Costa Aguiar Alves, Ligia Ajaime Azzalis, and Thaís Moura Gáscon Belardo
