Part I: Quantifying the Mechanical Properties of Cells and Adhesion Proteins
1. Measuring Cell Mechanical Properties Using Microindentation
Julien Husson
2. In Situ Measurements of Cell Mechanical Properties Using Force-Spectroscopy
Meital Reches
3. Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy
Martin Bergert and Alba Diz-Muñoz
4. Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single Molecule Atomic Force Microscopy
Andrew Vae Priest and Sanjeevi Sivasankar
Part II: Exposing Cells to External Mechanical Forces
5. Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber
Jason Sedlak and Alisa Clyne
6. Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching
Robin Püllen, Jens Konrad, Bernd Hoffmann, and Rudolf Merkel
7. Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo
Kenji Nishizawa, Claire Chardès, Raphaël Clément and Pierre-François Lenne
Part III: Quantitative Characterization of Cell Mechanosensing
8. A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity-Sensing by Plant's Roots and Root Hair Cells
David Pereira, Thomas Alline, Gaurav Singh, Marie-Edith Chabouté, and Atef Asnacios
9. Photoresponsive Hydrogels for Studying Mechanotransduction of Cells
Keun-Young Park, David J. Odde, and Mark D. Distefano
10. Analyzing Mechanically Evoked Currents at Cell-Substrate Junctions
Surabhi Shrestha, Jessica Richardson, and Kate Poole
11. Quantifying Strain Sensing Protein Recruitment During Stress Fiber Repair
Shailaja Seetharaman, Stefano Sala, Margaret L. Gardel, and Patrick W. Oakes
Part IV: Measuring Forces Applied by Cells on the Extracellular Matrix
12. Quantification of Invadopodia Formation and Matrix Degradation Activity
Jubina Balan Venghateri and Benjamin Geiger
13. Measuring Cellular Traction Forces with Micropillar Arrays
Malak Amer and Haguy Wolfenson
14. Imaging Cell Adhesive Force at the Single Molecule Level
Ying Tu and Xuefeng Wang
Part V: Force Measurement Inside Cells
15. Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM
Lukas Win
