Part I: Association of Transmembrane Helices
1. Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments
Lidia Cristian and Yao Zhang
2. Measurement of Transmembrane Peptide Interactions in Liposomes Using Förster Resonance Energy Transfer (FRET)
Ambalika Khadria and Alessandro Senes
3. Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping
Heedeok Hong, Yu-Chu Chang, and James U. Bowie
4. Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes
Lydia Tome, Dominik Steindorf, and Dirk Schneider
Part II: Interactions with the Lipid Bilayer
5. Analyzing the Effects of Hydrophobic Mismatch on Transmembrane α-Helices Using Tryptophan Fluorescence Spectroscopy
Gregory A. Caputo
6. Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure
Heather E. Findlay and Paula J. Booth
7. Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes
Andrei L. Lomize and Irina D. Pogozheva
Part III: NMR Methods
8. Membrane Protein Structure Determination: Back to the Membrane
Yong Yao, Yi Ding, Ye Tian, Stanley J. Opella, and Francesca M. Marassi
9. On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides
Fernando Porcelli, Ayyalusamy Ramamoorthy, George Barany, and Gianluigi Veglia
Part IV: Engineering Approaches
10. Prediction and Design of Outer Membrane Protein-Protein Interactions
Vikas Nanda, Daniel Hsieh, and Alexander Davis
11. Design of Transmembrane Peptides: Coping with Sticky Situations
Arianna Rath and Charles M. Deber
12. Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors
Matthew W. Lluis and Hang Yin
13. Fluorination in the Design of Membrane Protein Assemblies
Vijay M. Krishnamurthy and Krishna Kumar
