Preface...
Table of Contents...
Contributing Authors...
Part I Fundamentals
1. Metabolic Profiling: Topic, Challenges, Perspective
Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson
2. Quality Control and Validation Issues in LC-MS Metabolomics
Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson
3. Data Treatment for LC-MS Untargeted Analysis
Samantha Riccadonna and Pietro Franceschi
4. Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis
Michael Witting
Part II Methods
5. HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications
Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis
6. Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition
Filippos Michopoulos
7. LC-MS Untargeted Analysis
Elizabeth Want
8. NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts
Dimitra Benaki and Emmanuel Mikros
9. Untargeted GC-MS Metabolomics
Μatthaios-Εmmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa
10. Rat Fecal Metabolomics Based Analysis
Olga Deda, Helen G. Gika, and Georgios A. Theodoridis
11. GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
Petr Husek, Zdeněk Svagera, Dagmar Hanzlíková, Iva Opekarová, Lucie Řimnáčová, Helena Zahradníčková, and Petr Simek
12. Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples
Rawi Ramautar
Part III Plant/Food and Other Applications
13. Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR
Jan Schripsema and Denise Dagnino
14. NMR Spectroscopy Protocols for Food Metabolomics Applications
Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros
15. Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study
Brian Farneti
16. LC-MS Untargeted Protocol for the Analysis of Wine
Panagiotis Arapitsas and Fulvio Mattivi
Part IV Life Science Applications
17. Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage
Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li
18. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)
Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, and Serge Rudaz
19. Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation
Aikaterina Siopi and Vassilis Mougios