1. Construction of Small-Insert and Large-Insert Metagenomic Libraries
Carola Simon and Rolf Daniel
2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
Jacqueline Hollensteiner, Franziska Wemheuer, Dominik Schneider, Birgit Pfeiffer, and Bernd Wemheuer
3. Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds
Nancy Weiland-Bräuer, Livia Saleh, and Ruth A. Schmitz
4. Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA
Supathep Tansirichaiya, William Hutton, and Adam P. Roberts
5. Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next Generation Sequencing Approach
Alinne L.R. Santana-Pereira
6. Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes
Nicole Adam-Beyer and Mirjam Perner
7. Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity
Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel
8. Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities
Aranksha Thakor, Jiujun Cheng, and Trevor C. Charles
9. Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures
Jascha F.H. Macdonald, Ines Krohn, and Wolfgang R. Streit
10. The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes
Jennifer Chow, Pablo Pérez-García, Robert F. Dierkes, Hongli Zhang, and Wolfgang R. Streit
11. High-Throughput Screening for Thermostable Polyester Hydrolases
Yannick Branson, Christoffel P. S. Badenhorst, Lara Pfaff, Carolin Buchmann, Ren Wei, and Uwe T. Bornscheuer
12. Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System
Yuchen Han, Robert F. Dierkes, and Wolfgang R. Streit
13. Assigning Functions of Unknown Enzymes by High Throughput Enzyme Characterization
Patricia Molina, Laura Fernandez-Lopez, Peter N. Golyshin, and Manuel Ferrer
14. Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation
Ines Friedrich and Robert Hertel
15. CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis
Katharina Kohm, Veronika Theresa Lutz, Ines Friedrich, and Robert Hertel
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