1. Introduction to Membrane Lipids
Richard M. Epand
2. Introduction: Membrane properties (Good) for Life
Marek Cebecauer
3. Introduction To Fluorescence Probing Of Biological Membranes
Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mély
4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)
Charles Cranfield, Sonia Carne, Boris Martinac, and Bruce Cornell
5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells
Deborah A. Brown
6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning
KR Levental and I Levental
7. Asymmetric Giant Lipid Vesicle Fabrication
Peichi C. Hu and Noah Malmstadt
8. Cholesterol Depletion using Methyl-b-cyclodextrin
Saleemulla Mahammad and Ingela Parmryd
9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems
G. Ekin Atilla-Gokcumen and Ulrike S. Eggert
10. Imaging Membrane Order using Environmentally Sensitive Fluorophores
G Ashdown and DM Owen
11. 3D Super-resolution Imaging by Localisation Microscopy
Astrid Magenau and Katharina Gaus
12. Electron Microscopy Methods for Studying Plasma Membranes
Alison J. Beckett and Ian A. Prior
13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy
Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas
14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order
Richard KP Benninger
15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes
Eduard Hermann, Jonas Ries, and Ana J. García-Sáez
16. X-ray Diffraction of Lipid Model Membranes
Arwen I.I. Tyler, Robert V. Law, and John M. Seddon
17. Solid State NMR of Lipid Model Membranes
Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law
18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications
Michael Carnell, Alex Macmillan, and Renee Whan
19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells
Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton
20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations
Semen O. Yesylevskyy and Alexander P. Demchenko
21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers
Svetlana Baoukina and D. Peter Tieleman