Part I. Investigations on Biofilms in Health and Disease
1. Methods for Dynamic Investigations of Surface Attached in vitro Bacterial and Fungal Biofilms
Claus Sternberg, Thomas Bjarnsholt, and Mark Shirtliff
2. Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms
Mohammed Dwidar, Shuichi Takayama, and Robert J. Mitchell
3. Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and enterococcal fsr Systems
Akane Shojima and Jiro Nakayama
4. Advanced Techniques for in situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy
Thomas R. Neu and John R. Lawrence
5. Multiplex Fluorescence in situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyse Multispecies Oral Biofilms
Lamprini Karygianni, Elmar Hellwig, Ali Al-Ahmad
6. Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial Infections
Claudia Vuotto and Gianfranco Donelli
7. Experimental Approaches to Investigating the Vaginal Biofilm Microbiome
Marc M. Baum, Manjula Gunawardana, and Paul Webster
8. Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopaedic Infections
L. Nistico, L. Hall-Stoodley, and P. Stoodley
9. Animal Models to Evaluate Bacterial Biofilm Development
Kim Thomsen, Hannah Trøstrup, and Claus Moser
10. Animal Models to Investigate Fungal Biofilm Formation
Jyotsna Chandra, Eric Pearlman, and Mahmoud A. Ghannoum
11. Non-Mammalian Model Systems to Investigate Fungal Biofilms
Marios Arvanitis, Beth Burgwyn Fuchs, and Eleftherios Mylonakis
Part II. Investigations on anti-biofilm compounds and strategies
12. Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors
Livia Leoni and Paolo Landini
13. In vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development
Marion Girardot and Christine Imbert
14. Biofilm Matrix-Degrading Enzymes
Jeffrey B. Kaplan
15. Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices
Iolanda Francolini, Antonella Piozzi, and Gianfranco Donelli
16. Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy
Wean Sin Cheow and Kunn Hadinoto
17. Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa in vitro and in vivo
Wang Hengzhuang, Niels Høiby, and Oana Ciofu
18. Contribution of Confocal Laser Scanning Microscopy in Deciphering Biofilm Tridimensional Structure and Reactivity
Arnaud Bridier and Romain Briandet
19. Chip-Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics and Biological Agents
Frida Mariana, Friederike Buchholz, and Thomas Maskow
20. Bacteriophage Attack as an Anti-Biofilm Strategy
Sanna Sillankorva and Joana Azeredo
21. Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-Based Nosocomial Infections: Study Protocols
Francesco Giuliani
Part III. Investigations on biofilms in the environment and manufacturing plants
22. Capturing Air-Water Interface Biofilms for Microscopy and Molecular Analysis
Margaret C. Henk
23. Biofilm-Growing Bacteria Involved in the Corrosion of Concrete Wastewater Pipes: Protocols for Comparative Metagenomic Analyses
Vicente Gomez-Alvarez
24. Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Cultural Heritage Stone Materials and Frescoes
Francesca Cappitelli, Federica Villa, and Andrea Pol
