1. Pathway and Gene Discovery from Natural Hosts and Organisms
Jing Ke and Yasuo Yoshikuni
2. Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity
James Jeffryes, Jonathan Strutz, Chris Henry, and Keith E.J. Tyo
3. Antisense RNA Elements for Downregulating Expression
Yaping Yang, Jian Wang, Ruihua Zhang, and Yajun Yan
4. Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering Aaron M. Love, Bradley W. Biggs, Keith E.J. Tyo, and Parayil Kumaran Ajikumar
5. Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs
Demetrios Maroutsos, Hannah Huff, and Aditi Das
6. Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae
Shuobo Shi, Youyun Liang, Ee Lui Ang, and Huimin Zhao
7. Assembly of Complex Pathways using Type IIs Restriction Enzymes
Sylvestre Marillonnet and Stefan Werner
8. Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly D. Bauwens, P. Coussement, J. Maertens, and M. De Mey
9. Checks and Balances with Use of the Keio Collection for Phenotype Testing
Sandra J. Aedo, Helena R. Ma, and Mark P. Brynildsen
10. Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803
Jacob Sebesta, Allison Zimont, and Christie Ann Marie Peebles
11. Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica
Lynn Wong, Benjamin Holdridge, Jake Engel, and Peng Xu
12. Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization
McKenzie L. Smith, April M. Miguez, and Mark P. Styczynski
13. Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization Hsine-Chung Tseng and Christine Nicole S Santos
14. A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models
Sriram Chandrasekaran
15. 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms
Whitney Hollinshead, Lian He, and Yinjie J. Tang
16. A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast Manisha Wadhwa andAnand K Bachhawat
